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不同浓度NAA对锦灯笼诱导成活率的影响
引用本文:奚广生,申延国.不同浓度NAA对锦灯笼诱导成活率的影响[J].安徽农业科学,2010,38(1):51-51,57.
作者姓名:奚广生  申延国
作者单位:吉林农业科技学院,吉林吉林,132100;吉林省桦甸市农业技术推广中心,吉林桦甸,132400
基金项目:吉林省教育厅项目(2007423)
摘    要:目的]为锦灯笼的快繁技术提供参考。方法]以锦灯笼茎尖为外植体,NAA浓度分别为0.1、0.3、0.5mg/L,研究不同浓度NAA对锦灯笼诱导成活率的影响。结果]不同浓度NAA对锦灯笼诱导成活率的影响不同,以处理③(MS+NAA0.5mg/L+6-BA0.3mg/L+琼脂8g/L+蔗糖30g/L)的诱导效果最佳,诱导成活率达93.3%;其次为处理②(Ms+NAA0.3mg/L+6-BA0.3mg/L+琼脂8g/L+蔗糖30g/L),诱导率为63.3%;处理①(Ms+NAA0.1mg/L+6-BA0.3mg/L+琼脂8g/L+蔗糖30g/L)的诱导率最低,仅43.3%。结论]处理③是最佳诱导分化培养基。

关 键 词:锦灯笼  NAA  诱导成活率

Effect of Different NAA Concentrations on Survival Rate of Induction of Franchet Groundcherry Calyx
XI Guang-sheng et al.Effect of Different NAA Concentrations on Survival Rate of Induction of Franchet Groundcherry Calyx[J].Journal of Anhui Agricultural Sciences,2010,38(1):51-51,57.
Authors:XI Guang-sheng
Institution:Jilin Agricultural Science and Technology College;Jilin;Jilin 132100
Abstract:Objective] The study aimed to provide the references on rapid propagation technique of Franchet Groundcherry Calyx. Method]With stem top of Franchet Groundcherry Calyx as explant, concentration of NAA was set at 0.1, 0.3, 0.5 mg/L, the effects of different NAA concentration on induced survival rate were studied. Method] Different NAA concentration had different effect on induced survival rate. The effect of treatment ③ (MS+NAA 0.5 mg/L + 6-BA 0.3 mg/L + agar 8 g/L+ sucrose 30 g/L) was the best, and its induced survival rate reached 93.3%; secondly for treatment ②, its induced survival rate was 63.3%; treatment ① had the lowest induced survival rate, which was 43.3%.Conclusion] Treatment ③ was the best inductive differential medium.
Keywords:Franchet Groundcherry Calyx  NAA  Induced survival rate  
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