首页 | 本学科首页   官方微博 | 高级检索  
     检索      

虎杖中白藜芦醇苷不同检测方法的比较
引用本文:陈爽,周国海,皮小武,贺丽萍,曹庸.虎杖中白藜芦醇苷不同检测方法的比较[J].安徽农业科学,2012(5):2659-2660,2663.
作者姓名:陈爽  周国海  皮小武  贺丽萍  曹庸
作者单位:吉首大学林产化工工程湖南省重点实验室吉首大学张家界校区,湖南张家界,427000;吉首大学林产化工工程湖南省重点实验室吉首大学张家界校区,湖南张家界 427000;泰州师范高等专科学校,江苏泰州 225300;华南农业大学食品学院,广东广州,510642
摘    要:目的]分析比较不同方法测定的人工栽培虎杖中白藜芦醇苷的含量差异。方法]采用高效液相色谱法(HPLC)和毛细管电泳法(CE)对人工栽培虎杖中白藜芦醇苷进行测定,并对这2种方法的线性范围、定量重现性、最低检测限及分离分析时间进行比较研究。结果]选定HPLC-FD为测虎杖中白藜芦醇苷的最佳检测方法。检测条件:色谱柱为Diamonsil-C18(250 mm×0.46 mm,5μm),激发波长为334 nm,发射波长为408 nm,流动相为浓度25%乙腈,流速为1.0 ml/min,柱温为25℃,进样量为10μl。结论]高效液相色谱法是测定虎杖中白藜芦醇苷含量较理想的方法。

关 键 词:虎杖(RHIZOMA  ET  RADIX  POLYGONI  CUSPIDATI)  白藜芦醇苷  高效液相色谱  毛细管电泳

Comparison of Different Methods for the Detection of Polydatin in RHIZOMA ET RADIX POLYGONI CUSPIDATI
Institution:CHEN Shuang et al(Hunan Provincial Key Laboratory of Forest Product and Chemical Industry Engineering,Jishou University,Zhang jiajie,Hunan 427000)
Abstract:Objective] To compare the content of polydatin in artificial cultivated RHIZOMA ET RADIX POLYGONI CUSPIDATI determined by different methods.Method] High-performance liquid chromatography(HPLC) and capillary electrophoresis(CE) were adopted to determine the content of polydatin in the artificial cultivars of RHIZOMA ET RADIX POLYGONI CUSPIDATI.Then,the linear range,quantitative reproducibility,detection limit and analysis time of the separation between the two methods were comparatively studied.Result] High-performance liquid chromatography with fluorescence detection(HPLC-FD) was the optimum method for detecting polydatin in RHIZOMA ET RADIX POLYGONI CUSPIDATI.Following the injection of 10 μl of sample solution,polydatin was separated on a chromatographic column Diamonsil-C18(250 mm×0.46 mm,5 μm) with 25% acetonitrile as mobile phase at the column temperature of 25 ℃,and then quantified by fluorescence detection with the excitation wavelength at 334 nm and the emission wavelength at 408 nm.The flow rate of mobile phase was set to 1.0 ml/min.Conclusion] HPLC is an ideal method to determine the content of polydatin in RHIZOMA ET RADIX POLYGONI CUSPIDATI.
Keywords:RHIZOMA ET RADIX POLYGONI CUSPIDATI  Polydatin  HPLC  CE
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号