广西沼泽型母水牛发情淡季生殖激素的变化规律研究

目的]研究发情淡季母水牛生殖激素的变化规律及各激素之间的相互关系。方法]采用酶联免疫分析方法(ELISA)测定了发情淡季广西沼泽母水牛血样中促卵泡素(FSH)、促黄体素(LH)、抑制素(INH)、雌二醇(E2)和孕酮(P4)的浓度变化,并结合B超技术检测了母水牛卵泡在发情周期中的变化。结果]广西沼泽型母水牛血样中E2和P4浓度呈波动式变化。其中,E2分别在排卵后第13天d下降到基础水平。FSH在排卵后的第10天出现峰值;LH在排卵前2天出现峰值;INH在排卵后第4天出现峰值。各种激素之间存在相关性。FSH、LH和INH三者两两之间存在极显著相关性,INH与E2、P4存在显著相关性,E2与P4存在极显著相关性。结论]该研究可为指导适时配种、提高广西本地沼泽型母水牛的繁殖力提供理论参考。

Variations of Reproductive Hormones of Female Swamp Buffalos from Guangxi Region in Anestrus

Objective]The aim of the paper is to find the change law of reproductive hormones of female swamp buffalos in anestrus and to investigate the relationship between various hormones.Method]The concentrations of follicle stimulating hormone(FSH),luteinizing hormone(LH),inhibin(INH),estradiol(E2) and progesterone(P4) were determined by the enzyme-linked immunosorbent assay(ELISA) in anestrus.The changes of follicles in the estrous cycle were examined using B-mode ultrasonography.Result]In anestrus of female swamp buffalos from Guangxi region,the blood concentration of E2 and P4 varied fluctuantly.The concentration of E2 had two peaks,respectively 3 d before ovulation and after 13 d post ovulation.The concentration of P4 increased slowly from the first day after ovulation,increased rapidly from 5 to 13 d,peaked on Day 16 and decreased to the baseline 3 d before ovulation.The concentrations of FSH,LH and INH reached a peak respectively after 10 d post ovulation,2 d before ovulation,and after 4 d post ovulation.The changes of the hormones had correlations.The concentrations of FSH,LH and INH had highly significant correlations with each other,while the concentration of INH had highly significant correlations with that of E2 or P4.The concentration of E2 also had a highly significant correlation with that of P4.Conclusion]The study can guide the breeding of swamp buffalos from Guangxi region and also help to improve their reproduction performance.