柑橘皮多糖抗氧化性研究

目的]研究柑橘皮多糖的抗氧化活性。方法]利用水提醇沉法,提取柑橘皮多糖并进行初步纯化,采用苯酚-硫酸法测定柑橘皮多糖的提取率并采用水杨酸法研究柑橘皮多糖清除.OH的效果。结果]柑橘皮中多糖的含量为6.32%;不同浓度的柑橘皮多糖对H2O2/Fe2+体系通过Fenton法反应产生的.OH都具有清除作用,且随浓度的增加,清除率逐渐升高,即清除率与多糖的用量存在一定的量效关系。结论]柑橘皮多糖具有明显地抗氧化性,对.OH具有清除作用。

Antioxidation of Polysaccharides from PERICARPIUM CITRI RETICULATAE

Objective] To study on the antioxidation of polysaccharides from PERICARPIUM CITRI RETICULATAE.Method] Isolation and purification of polysaccharides from PERICARPIUM CITRI RETICULATAE were conducted by hot water extraction method.Extraction rate of polysaccharides from PERICARPIUM CITRI RETICULATAE was determined by phenol-sulfuric acid method.Clearance effect of ·OH was studied by salicylic acid method.Result] Polysaccharides content in PERICARPIUM CITRI RETICULATAE was 6.32%;polysaccharides from PERICARPIUM CITRI RETICULATAE at different concentrations had clearance effect on ·OH,which was produced by Fenton method.With the increase of concentration,the clearance rate gradually enhanced.In other words,there was certain dose-effect relationship between clearance rate and polysaccharides dosage.Conclusion] Polysaccharides in PERICARPIUM CITRI RETICULATAE had significant antioxidation and had clearance effect on ·OH.