| 不同白蚁基因组DNA提取方法的比较 |
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| 引用本文： | 张总泽,李,敏,林阳武等.不同白蚁基因组DNA提取方法的比较[J].安徽农业科学,2014(23):7714-7715. |
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| 作者姓名： | 张总泽 李 敏 林阳武等 |
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| 作者单位： | 福建出入境检验检疫局,福建福州350001 |
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| 基金项目： | 福建检验检疫局科技计划项目（FK2012-24）;质检公益性行业科研专项（201410076）. |
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| 摘 要： | 目的]筛选一种高效、简单、快速、价格合理的白蚁基因组DNA提取方法。方法]比较了CTAB法、离心柱试剂盒和磁珠法试剂盒提取白蚁基因组DNA的效果。结果]对于新鲜和保存得当的标本,3种方法提取的DNA质量均较好;但对于保存时间较长,出现降解的标本,磁珠法试剂盒的效果明显优于其他方法。结论]获得分子检测所需要的最佳DNA提取方法。
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| 关 键 词： | 白蚁 DNA 提取 |
Comparison of Genomic DNA Extracting Methods for Termites |
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| Institution: | ZHANG Zong-ze et al (Fujian Exit-Entry Inspection and Quarantine Bureau, Fuzhou, Fujian 350001 ) . |
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| Abstract: | Objective] The aim was to choose an efficient, simple, fast and affordable DNA extraction method. Method] The genome DNA of termites were extracted by using three methods, CTAB method, TIANamp genomic DNA kit and magnetic genomic DNA kit. Result] The results showed that three methods were effective, when the termites were fresh or properly saved. The extraction method of using magnetic ge- uomic DNA kit was much better than the other two methods, when the DNA of termites were breaking down. Conclusion] The best molecular detection DNA extraction method was gotton. |
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| Keywords: | Termites DNA Extract |
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