澳洲茶树黄酮提取研究

目的]优化澳洲茶树中黄酮类化合物的提取条件。方法]以澳洲茶树叶的总黄酮提取率为评价指标,通过单因素试验对回流提取澳洲茶树黄酮类化合物的工艺条件进行了研究,再运用正交试验确定最佳提取条件。结果]常规回流浸提法提取澳洲茶树中黄酮的优化条件为:溶剂使用75%(V/V)乙醇,料液比1∶25 g/ml,提取时间为2.0 h,浸提温度80℃,该优化工艺下提取率最高,为6.32%。结论]回流提取澳洲茶树中的黄酮类物质,此方法设备简单、成本低、操作简单易行,且对环境及人类无毒害,提取率较高,是一种理想的提取方法。

Reflux Extraction of Flavonoids from the Melaleuca ahemifolia

Objective ] To optimize the extraction conditions for flavonoids from Melaleuca alternifolia. Method J With total ilavonoids extraction rate of Melaleuca alternifolia as evaluation indexes, the technique for extracting flavonoids from Melaleuca alternifolia was studied by single-factor test, the optimal extraction conditions were determined by orthogonal test. Result] The optimal conditions were 75% （V/V） ethanol, solid-liquid ratio 1：25 （g/ml）, extraction time 2.0 h, extraction temperature 80℃, the maximum yield can up to 6.32%. Conclusion ] The method is simple, low cost, simple operation, and non-toxic to the environment and humans with higher extraction rate, which is an ideal extraction method.