暹罗斗鱼致病性嗜水气单胞菌的分离·鉴定及药敏试验

目的]探讨暹罗斗鱼的发病原因和病害防治方法。方法]对暹罗斗鱼的病原菌进行分离,并通过生理生化指标测定和16S rD-NA测序对其鉴定,同时进行药敏试验。结果]菌株DY001为暹罗斗鱼嗜水气单胞菌病的病原菌。经鉴定,确定菌株DY001为嗜水气单胞菌(Aeromonas hydrophila)。该病原菌对暹罗斗鱼的半致死量(LD50)为0.04×106CFU/g。药敏试验结果表明,病原菌对阿奇霉素、奥复星、氟哌酸、复方新诺明、菌必治、甲氧苄啶和氯霉素等抗生素敏感。结论]嗜水气单胞菌是引起此次暹罗斗鱼发病的病原菌,而药敏试验结果将为其防治提供参考。

Isolation, Identification and Drug Sensitivity Test of Pathogenic Aeromonas hydrophila from Betta splendens Regan

Objective] The research aimed to discuss the disease cause of Betta splenders Regan and the prevention and control methods of its diseases and pests.Method] The pathogenic bacteria of B.splenders were isolated and identified by determining the physiological and biochemical indices and 16 S rDNA sequencing.And the drug sensitivity test was made.Result] Strain DY001 was the pathogenic bacteria that caused Aeromonas hydrophila disease of B.splenders.Strain DY001 was identified as Aeromonas hydrophila.The median lethal dose(LD50) of this pathogeny to B.splenders was 0.04×106 CFU/g.The results of drug sensitivity test showed that this pathogenic bacteria was sensitive to azithromycin,ofloxacin,norfloxacin,compound sinomin,ceftriaxone,trimethoprim,chloramphenicol and other antibiotics.Conclusion] Aeromonas hydrophila was the pathogenic bacteria that caused the disease incidence of B.splenders.The results of drug sensitivity test could provide references for the disease prevention and control of B.splenders.