坛紫菜藻红蛋白分离纯化研究

目的]建立一种新型、高效、快速的藻红蛋白分离纯化方法,并对其分离机理进行探讨。方法]以坛紫菜的藻红蛋白搅切法提取物为原料,首先通过100和500 kDa的超滤离心管对其进行超滤分离,再在Superose 12色谱柱上以不同种类和不同浓度的流动相对藻红蛋白粗提物进行等度洗脱,分析其分离机理,并优化分离条件。结果]在0.05 mol/L NaCl等度洗脱的条件下,藻红蛋白在Superose12色谱柱上表现为典型的氢键吸附模式;在优化的条件下,通过超滤-Superose 12氢键吸附分离的方法,可以使坛紫菜藻红蛋白提取物纯度(A565/A280)达到7.98,总回收率52.9%。结论]该研究结果表明藻红蛋白在Superose 12色谱柱上存在氢键吸附作用,同时,该研究所建立的超滤-Superose 12氢键吸附分离方法是一种新型、高效、快速的藻红蛋白分离纯化方法。

Study on the Separation and Purification of Phycoerythrin from Porphyra haitanensis

Objective]To establish a new,efficient and rapid separation and purification method of phycoerythrin from P.haitanensis,and discuss its separation mechanism.Method]With the phycoerythrin extracts from P.haitanensis as the materials,they were separated by 100 and 500 kDa ultrafiltration tubes,and then eluted on Superose 12 using different kinds and different concentrations of solution as mobile phase,the separation mechanism and conditions were analyzed.Result]The retention mechanism of phycoerythrin on Superose 12 under 0.05 mol/L NaCl isocratic elute is typically a hydrogen bond adsorption pattern.Using the ultrafiltration method and Superose 12 hydrogen bond adsorption for the separation of phycoerythrin extracted from Porphyra haitanensis,the purity(A565/A280) could be up to 7.98 with a total recovery of 52.9%.Conclusion]There is hydrogen bond adsorption between Superose 12 and phycoerythrin.The ultrafiltration method and Superose 12 hydrogen bond adsorption for the separation of phycoerythrin is a new,efficient and rapid separation method.