响应面法优化葵花粕中绿原酸提取工艺

目的]优化葵花粕中绿原酸的超声波辅助提取工艺。方法]在单因素试验的基础上,选取乙醇体积分数、提取温度、提取时间为自变量,以绿原酸的提取率为响应值,利用响应面法对绿原酸提取条件进行优化。结果]葵花粕中绿原酸超声波辅助提取的最佳工艺条件为乙醇体积分数57%,提取时间39 min,提取温度53℃,超声功率100 W,料液比1∶14 g/ml,提取1次。在该条件下,绿原酸的提取率达2.25%。结论]超声波辅助提取法具有提取率高、时间短等特点,该研究为葵花粕绿原酸的工业化生产提供了理论依据。

Optimization of the Extraction Technology of Chlorogenic Acid from Sunflower Meal Using Response Surface Methodology

Objective] To optimize the ultrasonic-assisted extraction process of chlorogenic acid from sunflower meal.Method] Based on the single-factor test,the extraction factors of ethanol concentration,extraction temperature and extraction time were optimized using the response surface methodology with the extraction rate of chlorogenic acid as the response value.Result] The optimum extraction conditions of chlorogenic acid from sunflower meal were 57% of ethanol concentration,39 min of extraction time,53 ℃ of extraction temperature,100 W of ultrasonic power,1∶14 g/ml of solid-liquid ratio and one time of extraction.Under these conditions,the extraction rate of chlorogenic acid was 2.25%.Conclusion] Ultrasonic assisted extraction method has high extraction rate but short time,the study provides a theoretical basis for the industrialized production of chlorogenic acid in sunflower meal.