| 优化醋酸钾(KAC)法提取蚜虫基因组DNA |
| |
| 引用本文： | 赵丽娟,漆永红,刘永刚,张正英.优化醋酸钾(KAC)法提取蚜虫基因组DNA[J].安徽农业科学,2009,37(10):4394-4395. |
| |
| 作者姓名： | 赵丽娟 漆永红 刘永刚 张正英 |
| |
| 作者单位： | 甘肃农业科学院生物技术研究所,甘肃兰州,730070;甘肃农业科学院植物保护研究所,甘肃兰州,730070 |
| |
| 基金项目： | 甘肃省科技厅事业费(QS031-C31-15) |
| |
| 摘 要： | 目的]探索一种简便、有效的单头蚜虫基因组DNA提取方法。方法]以从不同地区采集的桃蚜为试材,采用改进的KAC法和优化的KAC法提取单头桃蚜的基因组DNA,用紫外分光光度计测定所提DNA的浓度（以OD260/280表示）,用0.8%琼脂糖凝胶电泳检测所提DNA的纯度,比较2种方法的提取效果;对优化KAC法所提DNA进行PCR扩增和电泳检测。结果]优化KAC法提取的单头蚜虫基因组DNA的OD260/280为1.6-1.9,浓度为20-50 ng/g;改进KAC法提取的单头蚜虫基因组DNA的OD260/280为1.4-1.7,浓度为8-25 ng/g;来自同一地方桃蚜的基因组DNA PCR扩增产物的电泳条带基本相同。结论]优化改进的KAC法提取的桃蚜基因组DNA浓度和纯度均较高。
|
| 关 键 词： | 基因组DNA 桃蚜 提取方法 优化 |
Extraction of Genome DNA of Aphid by Optimized Potassium Acetate (KAC) |
| |
| ZHAO Li-juan et al.Extraction of Genome DNA of Aphid by Optimized Potassium Acetate (KAC)[J].Journal of Anhui Agricultural Sciences,2009,37(10):4394-4395. |
| |
| Authors: | ZHAO Li-juan |
| |
| Institution: | ZHAO Li-juan et al(Insititute of Bio-technique,Gansu Academy of Agricultural Science,Lanzhou,Gansu 730070) |
| |
| Abstract: | Objective] The aim was to explore a simple and effective extraction method for extracting genome DNA of single aphid.Method] With peach aphids from different areas as the tested materials,the improved KAC method and optimized KAC method were taken to extract the genome DNA of single aphids.The concn.of obtained DNA(OD260/280) was determined by ultraviolet spectrophotometer and its purity was determined by 0.8% agarose gel electrophoresis.The extraction effects of 2 methods were compared.The DNA obtained b... |
| |
| Keywords: | Genome DNA Peach aphid Extraction method Optimization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
