虎雪兰与朱砂兰、蕙兰正反交育种及种子无菌萌发与增殖研究

目的]研究虎雪兰与朱砂兰、蕙兰正反交育种及种子无菌萌发与增殖.方法]以虎雪兰、朱砂兰、蕙兰‘绿春兰’为亲本,采用正反交杂交法进行育种试验得到果实,对成熟的种子进行无菌萌发试验,并对种子萌发获得的小苗进行增殖研究.结果]朱砂兰为母本获得杂交种子无菌萌发最适培养基为：1/2MS ＋0.5 mg/L BA ＋0.2 mg/L NAA ＋3％花宝1号＋0.05％碳粉;最佳增殖及生长培养基为：1/2MS＋1.0 mg/L 6-BA ＋0.5 mg/L NAA ＋0.5 mg/L KT ＋3％花宝4号＋0.05％碳粉.蕙兰‘绿春兰’为母本获得种子萌发最适培养基为：1/2MS＋ 1.0 mg/L 6-BA ＋0.5 mg/L NAA＋ 3％花宝1号＋0.05％碳粉;最佳增殖及生长培养基为1/2MS＋ 1.5 mg/L 6-BA ＋0.5 mg/LNAA ＋0.5 mg/L KT＋3％花宝4号＋0.05％碳粉.结论]该方法为虎雪兰的种质资源栽培提供了理论依据.

Research on Aseptic Budding and Fast Propagation on Cymbidium tracyanum var huanghua × Cymbidium mastersii Interspecific Hybridization Seed

Objective] This paper aims to study the aseptic budding and fast propagation on Cymbidium tracyanum var huanghua × Cymbidium mastersii interspecific hybridization seed.Method] Cymbidium tracyanum var huanghua,Cymbidium mastersii and Cymbidium faberi Rolfe ‘ Lv Chun Lan’ were employed as parents.The hybrid seeds were tested using direct crossing and reciprocal crossing,then germinated and compared with different medium in axenic culture.Result] The results show that the best medium for the seeds of Cymbidium mastersii in axenic culture is 1/2MS ＋ 0.5 mg/L BA ＋0.2 mg/L NAA ＋ 3％ Hyponex 1 ＋ 0.05％ C,the best medium for multiplication culture and differentiation is 1/2MS ＋ 1.0 mg/L 6-BA ＋ 0.5 mg/L NAA ＋ 0.5 mg/L KT ＋ 3 ％ Hyponex 4 ＋ 0.05％ C.The medium for the seeds of Cymbidium faberi Rolfe ‘ Lv Chun Lan’ in axenic culture is 1/2MS ＋ 1.0mg/L 6-BA ＋0.5 mg/L NAA ＋3％ Hyponex 1 ＋0.05％ C,the best medium for multiplication culture and differentiation is 1/2MS ＋ 1.5 mg/L 6-BA ＋0.5 mg/L NAA ＋0.5 mg/L KT ＋3％ Hyponex ＋0.05％C.Conclusion] This method provides a theoretical basis for the germplasm resource cultivation of Cymbidium tracyanum var huanghua ×Cymbidium mastersii.