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| 反义CCoAOMT基因调控烟草木质素的生物合成 | |
| 引用本文： | 黄春琼,刘国道,郭安平.反义CCoAOMT基因调控烟草木质素的生物合成[J].安徽农业科学,2008,36(19). |
| 作者姓名： | 黄春琼 刘国道 郭安平 |
| 作者单位： | 1. 中国热带农业科学院热带作物品种资源研究所,海南儋州,571737;中国热带农业科学院热带生物技术研究所,海南海口,571101;海南大学农学院,海南儋州,571737 2. 中国热带农业科学院热带作物品种资源研究所,海南儋州,571737 3. 中国热带农业科学院热带生物技术研究所,海南海口,571101 |
| 摘 要： | 目的]研究木质素的生物合成及调控,降低木质素的含量或改变其组分。方法]用农杆菌介导法将苎麻反义CCoAOMT基因导入烟草,采用PCR及Southern印迹对获得的转基因植株进行分子检测,并测定移栽3个月转基因植株Klason木质素及纤维素含量。结果]转基因烟草与野生型对照相比木质素平均含量降低了16.8%,纤维素平均含量升高了15.2%,并且植株生长正常。结论]抑制植物CCoAOMT表达是反向调控转基因植物木质素生物合成的有效途径。 |
| 关 键 词： | 苎麻 CCoAOMT基因 反义RNA转化 木质素 |
Lignin Biosynthesis Regulated by Antisense CCoAOMT Gene in Tobacco |
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| HUANG Chun-qiong et al.Lignin Biosynthesis Regulated by Antisense CCoAOMT Gene in Tobacco[J].Journal of Anhui Agricultural Sciences,2008,36(19). | |
| Authors: | HUANG Chun-qiong |
| Abstract: | Objective]The research aimed to study biosynthesis and regulation of lignin,reduce the content of lignin or change the composition of it.Method] The antisense CCoAOMT was transformed into tobacco by Agrobacterium tumefaciens mediated transformation.Transgenic plants were identified with PCR and Southern Blot.Lignin content and cellulose content in 3-month-old transgenic plants was measured.Result] The results showed that the lignin content in the transgenic tobacco reduced 16.8% and the cellulose content in the transgnnic tobacco increased 15.2% compared to untransformed tobacco.And the transgenic tobacco could grow well.Conclusion] It was an effective way to modulate the lignin biosynthesis by antisense CCoAOMT. |
| Keywords: | Boehmeria nivea CCoAOMT gene Antisense RNA transformation Lignin |
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