| 胞外表达L-天冬酰胺酶发酵条件的研究 |
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| 引用本文： | 王利群,黄达明,赵希岳,王文兵,徐苑.胞外表达L-天冬酰胺酶发酵条件的研究[J].安徽农业科学,2009,37(33). |
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| 作者姓名： | 王利群 黄达明 赵希岳 王文兵 徐苑 |
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| 作者单位： | 江苏工业学院化学化工学院,江苏常州,213164;江苏大学生命科学研究院,江苏镇江,212013 |
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| 基金项目： | 常州市科技局项目，镇江市科技局项目 |
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| 摘 要： | 目的]优化胞外表达L-天冬酰胺酶工程菌的发酵条件。方法]用正交试验研究培养基的组成,用单因素试验研究诱导条件以及通气量对酶产量的影响。结果]发酵培养基采用8.0%玉米浆和1.0%甘油组成;诱导剂采用终浓度为0.5g/L的乳糖,扩大培养后10h加入,诱导时间为12h,通气量为0.83VVM。结论]该研究为胞外表达L-天冬酰胺酶的工业化生产提供了参考。
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| 关 键 词： | L-天冬酰胺酶 胞外表达 发酵 |
Study on the Fermentation Conditions of Extracellular Expression L-asparaginase |
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| WANG Li-qun et al.Study on the Fermentation Conditions of Extracellular Expression L-asparaginase[J].Journal of Anhui Agricultural Sciences,2009,37(33). |
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| Authors: | WANG Li-qun |
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| Institution: | WANG Li-qun et al(Faculty of Chemistry & Chemical Engineering,Jiangsu Polytechnic University,Changzhou,Jiangsu 213164) |
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| Abstract: | Objective] The research aimed to optimize the fermentation conditions of the engineering bacteria that secreted L-asparaginase into media.Method] The ingredients of media were studied by orthogonal design.The effects of inducing conductions and aeration flow on enzyme production were studied by single-factor design.Result] Fermentation media was composed of 8.0% corn steep liquor and 1.0% glycerol.0.5 g/L lactose was added into media after culture 10 h to induce protein expression for 12 h.An appropriate... |
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| Keywords: | L-asparaginase Extracellular expression Fermentation |
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