大麻染色体制片技术

目的]探究大麻染色体的制片技术。方法]对大麻染色体制片技术的预处理液、解离时间、染色时间等进行试验研究,并对其进行染色体数目观察。结果]研究表明:用大麻根尖在常温下用0.003%的8-羟基喹啉溶液预处理2 h或用饱和对二氯苯处理1.5 h,经卡诺固定液固定30 min后,在1∶1的1 mol/L HCl和45%醋酸60℃下恒温水浴中解离30 s,地衣红染液染色8 h压片镜检,能取得良好的制片效果。大麻染色体数目观察表明,大麻染色体数目2n=2x=20。结论]该研究为大麻细胞学方面的深入研究提供了资料。

Study on the Squashing Technique of Chromosome in Cannabis sativa L

Objective] The research aimed to discuss the squashing technique of chromosome in Cannabis sativa L.Method] The pretreatment solution,the dissociation time and dyeing time in squashing technique of chromosome in C.sativa were studied.And its chromosome number was also observed.Result] Under room temperature the root tips of C.sativa were pretreated with 0.003 % 8-hydroxyquinoline solution for 2 h or treated with saturated p-dichlorobenzene for 1.5 h.After fixing with Carnoy stationary liquid for 30 min,they were dissociated in constant-temperature water bath of mixing 1 mol/L HCl with 45% acetic acid at 1∶1 for 30 s at 60 ℃ and stained with orcein dyeing solution for 8 h for squashing and microscopic examination.Thus good squashing effects could be obtained.The observation on the chromosome number of C.sativa indicated that the chromosome number of C.sativa was 2n=2x=20.Conclusion] This research provided data for in-depth study on C.sativa from the cytological aspect.