不同牛血清促悬浮培养BHK21细胞生长的作用

目的]验证成年牛血清在细胞培养过程中对细胞的促生长作用。方法]分别以商品胎牛血清、新生牛血清、处理的成年牛血清进行悬浮培养BHK21细胞,通过细胞密度、细胞活力等指标来检测各血清对细胞的促生长作用。结果]3种血清对细胞的促生长作用差异不明显,都具有良好的促细胞生长作用。结论]处理的成年牛血清具有良好的促细胞生长作用,与胎牛血清、新生牛血清促细胞生长作用差异不明显。

The Effect of Different Bovine Serum on Promoting the Growth of BHK21 Cells in Suspension Culture

Objective] To verify the effect of bovine serum on promoting cells growth during cultivation process.Method] Suspension culture of BHK21 cells was conducted by using commodity fetal bovine serum,newborn bovine serum and processed adult bovine serum respectively.The effect of each serum on promoting cells growth was tested through indicators of cell density and cell vitality.Result] The difference among three kinds of serum on cell growth was not obvious,and they all had good effects on promoting cell growth.Conclusion] The processed adult bovine has good effect on promoting cell growth,and has no obvious differences with that of fetal bovine serum and newborn bovine serum.