| 美味牛肝菌合成漆酶的发酵条件研究 |
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| 引用本文： | 陈今朝,王剑锋,王慧超,谭永忠.美味牛肝菌合成漆酶的发酵条件研究[J].安徽农业科学,2011(11):6543-6544. |
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| 作者姓名： | 陈今朝 王剑锋 王慧超 谭永忠 |
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| 作者单位： | 长江师范学院生命科学与技术学院;东华理工大学生物系; |
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| 基金项目： | 重庆市教委科研项目(KJ101304) |
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| 摘 要： | 目的]研究美味牛肝菌合成漆酶的最适发酵条件。方法]采用液体深层培养法研究不同碳源、氮源、pH、温度等因素对美味牛肝菌产酶量的影响。结果]美味牛肝菌合成漆酶的最适发酵条件为：葡萄糖2.0%,牛肉膏0.5%,KH2PO4 0.4%,MgSO4.7H2O 0.3%,微量元素溶液8.0%,维生素B1 0.003%,pH 4.5,温度28℃,培养时间为8 d。结论]在此条件下,漆酶产量最高,为71.27 U/ml,该研究为美味牛肝菌漆酶的开发应用提供了科学依据。
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| 关 键 词： | 美味牛肝菌 漆酶 液体深层培养 |
Study on Synthesis Conditions of Laccase from Boletus edulis |
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| CHEN Jin-zhao et al.Study on Synthesis Conditions of Laccase from Boletus edulis[J].Journal of Anhui Agricultural Sciences,2011(11):6543-6544. |
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| Authors: | CHEN Jin-zhao |
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| Institution: | CHEN Jin-zhao et al(College of Life Science and Technology,Yangtze Normal University,Chongqing 408100) |
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| Abstract: | Objective] The aim was to study the optimum fermentation conditions for the synthesis of laccase from Boletus edulis.Method] By using the submerged culture method,the effect of different carbon source,nitrogen source,pH and temperature on the laccase production from Boletus edulis.Result] The optimum synthesis conditions of laccase from Boletus edulis were as follows: glucose 2.0%,beef extract 0.5%,KH2PO4 0.4%,MgSO4·7H2O 0.3%,trace element solution 8%,Vitamin B1 0.003%,pH 4.5,temperature 28 ℃ and culture time 8 d.Conclusion] Under these conditions,the maximum laccase production was 71.27 U/ml,the study provided scientific basis for the development and application of laccase from Boletus edulis. |
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| Keywords: | Boletus edulis Synthesis of laccase Submerged culture |
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