| 人白介素-12转基因马铃薯中hIL-12的表达 |
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| 引用本文： | 陈佳瑜,罗果,葛正龙.人白介素-12转基因马铃薯中hIL-12的表达[J].安徽农业科学,2010,38(15):7767-7768. |
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| 作者姓名： | 陈佳瑜 罗果 葛正龙 |
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| 作者单位： | 遵义医学院生物化学教研室,贵州遵义,563003;遵义医学院中心实验室,贵州遵义,563003 |
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| 基金项目： | 贵州省科委重大攻关项目，贵州省教育厅重点项目 |
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| 摘 要： | 目的]研究人白介素-12转基因马铃薯中hIL-12的表达。方法]采用ELISA和WesternBlot,检测转基因马铃薯中hIL-12的表达情况。结果]转基因马铃薯叶与块茎中均能被hIL-12p70二聚体特异性的抗体识别的蛋白表达,与野生对照组相比均有0.01水平显著差异。WesternBlot分析结果显示,在40kD处有特异性的蛋白条带出现。结论]所建立的人白介素-12转基因马铃薯表达体系能正确、有效表达hIL-12二聚体。
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| 关 键 词： | 人白介素-12 表达 马铃薯 转基因植物 |
Human Interleukin 12 Expressed in Its Transgenic Potato Expression System |
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| Institution: | CHEN Jia-yu et al(Department of Biochemistry,Zunyi Medical College,Zunyi,Guizhou 563003) |
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| Abstract: | Objective] The research aimed to investigate the expression of human interleukin 12 in transgenic potatoes established by our research group.Method] The expressions of human interleukin 12 in transgenic potatoes were tested by ELISA and Western Blot.Result] human interleukin 12 production in the leaves and tubers was obviously higher than that of the wild type(P<0.01).Western blot analysis showed that the specific protein band presented at 40 kD.Conclusion] The human interleukin 12 was expressed in established transgenic potato expression system as heterodimers accurately. |
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| Keywords: | Human interleukin 12 Expression Potato Transgenic plant |
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