稀释液·清洁剂·冷冻程序对猪精液冷冻效果的影响

探讨了稀释液、清洁剂及冷冻程序对猪精液冷冻保存效果的影响。结果显示:①稀释液Ⅳ保存鲜精,精子活率明显低于其他组(P<0.05);但其作为冻精的解冻稀释液时,效果最佳(P<0.05),说明猪精液冷冻保存时可采用第Ⅰ～Ⅲ组稀释液,而解冻时采用第Ⅳ组较佳。②稀释液中于不同阶段添加SDS(Sodiumlaurylsulfate,十二烷基硫酸钠)或OEP(OrvusESPaste,清洁剂)均能显著提高冷冻精子活率(P<0.05),SDS与OEP对冻精活率的影响无显著差异(P>0.05)。③精液冷冻程序采用-70℃、液氮熏蒸与直接液氮熏蒸在对解冻后冻精精子活率上无显著差异(P>0.05)。添加咖啡因能有效提高冻精的解冻后活率。

Effect of the Extender, Detergent and Cooling Prolocols on Freezing Boar Sperm

The experiment in the effects of extender, detergent and cooling protocols on freezing boar semen was carried out. Fresh sperm were diluted by extendersⅠ～Ⅳ and enclosed in 5 ml straw. After placed in liquid nitrogen vapor, frozen sperm was thawed or stored for 3 h. The motility of sperm was detected. The result was following:①the motility in extender Ⅳ was lowest in four groups(at thawing: 60.0 %,57.2 %,56.3 % and 50.6 %,P<0.05; at 3 h storage: 17.9 %, 15.1 %, 13.5 % and 9.3 %,P<0.05); however, the extender Ⅳ was better than other groups as thawing dilution(at 3 h storage: 4.2 %, 6.8 %, 12.1 %, 32.8 %, P<0.05).②when detergent(SDS or OEP) were added to extender, the motility was promoted, but there was no significantly different between SDS and OEP.③ freezing protocols of 2-step cooling-down method(-70 ,-110 ℃) and 1-step cooling method(-110 ℃) had no effect on the percentage of motility after thawing. The addition of caffeine could promote the motility of frozen-thawed sperm.