降香叶不同提取部位体外抗氧化活性的比较

目的]考察降香叶不同提取部位的体外抗氧化活性,及其与黄酮类物质含量的相关性。方法]采用不同极性的溶剂对降香叶水提物进行萃取,得到石油醚、氯仿、乙酸乙酯和水等4个提取部位。以体外清除1,1-二苯基苦基苯肼(DPPH)自由基、过氧化氢、羟自由基和超氧阴离子的能力评价降香叶提取物的抗氧化活性;并采用铝盐络合法测定各个提取部位的黄酮含量,比较分析其含量与氧化能力的相关性;采用HPLC法分析其指纹。结果]降香叶各个提取部位均有不同程度的体外抗氧化能力,其效果顺序为:乙酸乙脂部位氯仿部位水提部位石油醚部位,与各部位黄酮含量呈正相关。结论]降香叶不同提取部位均具有抗氧化能力,其与黄酮类物质含量呈正相关,其乙酸乙脂部位可作为抗氧化活性部位。

In vitro Antioxidant Activity of the Extracts from Dalbergia Leaves

Objective] To evaluate the antioxidant activity of total flavones in different extracts from Dalbergia Odarifera leaves. Method ] The four extracts were prepared by aqueous extract of Dalbergia Odarifera leaves by 3 different organic solvents petroleum ether, chloroform, ethyl acetate and water. The antioxidant activities were assessed by DPPH radical scavenging activity, superoxide radical scavenging activity, hydroxyl radical scavenging activity, peroxide scavenging activity, and nitric oxide scavenging activity, with the positive control of the antioxi- dant activity of ascorbic acid. The contents of total flavones were measured by Aluminum salt complex method. Result] The pecking order of antioxidant capacity was Ethyl acetate parts 〉 Chloroform parts 〉 water extraction 〉 Petroleum ether parts, and consistent with the content of the flavonoids. Conclusion ] Antioxidant components could mainly exist in the ethyl acetate parts, the antioxidant capacity was associated with its flavonoids content.