秋水仙素对马尾松种子发芽的影响

目的]研究秋水仙素对马尾松种子发芽的影响。方法]研究不同浓度的秋水仙素对马尾松种子发芽及幼苗生理特性的影响。结果]当秋水仙素的浓度为60和80 mg/L时,种子的平均发芽时间明显延长,当秋水仙素的浓度大于60 mg/L时种子发芽后致畸率为37%,当秋水仙素的浓度高于80 mg/L时种子致死率为81.7%;经秋水仙素处理后的植株表现为针叶粗短,颜色加深,叶形指数减小,气孔变大,叶绿素含量、可溶性蛋白含量、可溶性糖含量均高于对照组。结论]该研究为马尾松突变体的产生筛选及良种选育提供了基础。

Effects of Colchicine on Seeds Germination of Pinus massoniana Lamb

Objective ] The paper was to study the effects of colchicine on seeds germination of Pinus massoniana Lamb.. Method] The effects of different concentrations of colchicine on the germination and seedling physiological characteristics of P. massoniana were studied. Result] When the concentrations of colchicine were 60 and 80 mg/L, the average germination times were significantly prolonged; when the concentration of colchicine was greater than 60 mg/L, the teratogenic rate after seed germination was 37% ; when the concentration of colchi- cine was higher than 80 mg/L, the lethality rate of seeds was up to 81.7%. Plants treated by colchicine showed the pine needles turned to be short and thick with deep colors and a decrease of leaf shape index, accompanied with air hole extended. The contents of chlorophyll, soluble protein and soluble sugar of treatment groups were higher than that of control group. Conclusion] The study provided foundation for the gener- ation and screening of P. massoniana mutants and the breeding of excellent varieties.