| 衍生化气相色谱法分析降解液中麦草畏含量的研究 |
| |
| 引用本文： | 靳魁奇,刘宇鹏,金文究,孙杨,潘龙,史欣诺.衍生化气相色谱法分析降解液中麦草畏含量的研究[J].安徽农业科学,2013,41(9):3864-3865. |
| |
| 作者姓名： | 靳魁奇 刘宇鹏 金文究 孙杨 潘龙 史欣诺 |
| |
| 作者单位： | 河南大学生命科学学院生物工程研究所,河南开封,475004;浙江杭州鑫富药业股份有限公司,浙江杭州,311300;中国药科大学生命科学与技术学院,江苏南京,211198 |
| |
| 基金项目： | 2011年度河南大学青年骨干教师培养计划项目，河南省教育厅自然科学基础研究计划项目 |
| |
| 摘 要： | 目的]建立衍生化气相色谱定量测定麦草畏的方法。方法]以五氟苄基溴为衍生剂,乙醚为萃取剂,正十六烷为内标物,使用DB-WAX毛细管柱和FID检测器对麦草畏含量进行测定,并首次对微生物降解液中麦草畏含量进行了测定。结果]在麦草畏浓度为0～240μg/ml时方法的线性回归方程为:y=3.957x+0.015,相关系数为0.999 0,线性关系良好。方法的相对标准偏差为1.51%,平均回收率在98.5%～101.8%,精密度和准确度较好。结论]该方法适合于微生物降解液中麦草畏含量的测定,能够满足检测工作的要求。
|
| 关 键 词： | 衍生化气相色谱 麦草畏 五氟苄基溴 降解 |
Determination of Dicamba in Degradation Liquid by Derivatization Gas Chromatography |
| |
| Institution: | JIN Kui-qi et al ( Institute of Biotechnology, School of Life Science, Henan University, Kaifeng, Henan 475004) |
| |
| Abstract: | Objective ] The aim was to establish an analytical method for the determination of dicamba based on derivatization gas chromatog- raphy. Method] The content of dicamba was determined by using DB-WAX capillary column and FID detector with PFBBr as derivative a- gent, ether as extraction agent and n-hexadecane as internal standard. Result] When the concentration of dicamba was 0 - 240 μg/ml, the linear regression equation was y = 3. 957x ＋ 0. 015, with the linear correlation coefficient of 0. 999 0. The relative standard deviation was 1.51%, and the recoveries ranged from 98.5% to 101.8%, which suggested that there were high precision and accuracy. Conclusion] The method was suitable for the determination of dicamba in microbial degradation liquid, and it could satisfy the requirement of determination a- nalysis. |
| |
| Keywords: | Derivatization gas chromatography Dicamba PFBBr Degradation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
