LC-MS/MS测定四大南药中黄曲霉毒素含量

目的]采用高效液相色谱-串联质谱仪（LC-MS/MS）,在多反应监测（MRM）模式下建立了四大南药中黄曲霉毒素（AFB1、AFB2、AFG1、AFG2）含量的测定方法.方法]试样中的黄曲霉毒素经甲醇/水（70/30）提取,黄曲霉毒素免疫亲和柱净化,液相色谱-串联质谱法测定,外标法定量.结果]该方法的检出限（LOD）为0.2～0.5 μg/kg,线性范围为0～50 μg/L,相关系数R2腰均大于0.999;在2～50μg/ks的添加水平上,4种霉菌毒素平均回收率为78.6％ ～92.1％,相对标准偏差RSD为0.8％ ～8.4％.结论]该方法前处理简单、快速,灵敏度、准确度和精密度高.

Determination of Aflatoxin contaminants in four south medicines by LC-MS/MS

Objective] The research aimed to develop the determination method of aflatoxin （AFB~ ,AFB2 ,AFG ,AFG2 ）content in four southmedicines in the multiple reaction monitoring （MRM） mode by high performance liquid chromatography-tandem mass spectrometry （LC-MS/MS）. Method ] The drugs were extracted with methanol/water（ 70/30, V/V）, and cleaned up by using beacon aflatoxin column, dissolved inmethanol and analysised by LC-MS/MS. Resuh~The limits of detection（LOD） were O. 2 -0.5 ixg/kg. The calibration curves were linear be-tween 0 to 50 txg/L, and the correlation coefficient was more than 0. 999. The average recoveries ranged from 78.6% - 92.1% and RSD was0.8% - 8.4% by addition of 2 - 50 p~g/kg avermectin standards to four south medicines as matrixes. Conclusion] The proposed method wasfast, simple, sensitive and accurate.