GC-μECD法快速检测猪肉地西泮残留

目的]利用气相色谱-微电子捕获（GC-μECD）检测法，快速检测猪肉中地西泮残留，为该类药物在动物性食品检测提供参考。方法]首先用乙腈超声提取猪肉中的地西泮，C18固相萃取柱净化，利用GC-μECD法进行残留检测，外标法定量。结果]在1-1000μg/L范围内药物色谱峰面积与浓度线性关系良好，相关系数达到0.9994。对空白猪肉进行1-50μg／kg范围的药物添加回收试验，平均回收率为83.5％-94.9％，日内变异系数为2.4％-8.1％，日间变异系数为3.9％-6.4％。检出限为0.31μ/kg，定量限为1μg/kg。结论]该方法简便、快速，稳定性好，灵敏度高，线性范围广，适用于猪肉中地西泮的残留检测。

Quick Determination of Diazepam in Pork by Gas Chromatograph-microelectron Capture Detection

Objective] The research aimed to detect diazepam residue in pork rapidly by gas chromatography with micro-electron capture detector method and provide reference for examining this kind of drug in the foord stuffs of animal origin.Method]Diazapam was extracted from pork by acetonitrile,cleaned up on C18 SPE column and subsequently detected by GC-μECD,and quantified with external standard method.Results] The concentration of drug from 1 to 1 000 μg/L to peak area showed good calibration curve with coefficient value of 0.999 4.When spiked with 1-50 μg/kg of diazepam,the recovery was 83.5%-94.9%,the inter-day RSD and intra-day RSD were between 2.4%-8.1% and 3.9%-6.4% respectively.The LOD and LOQ was 0.31 μg/kg and 1 μg/kg respectively.Conclusion]The method was simple,rapid,sensitive and was suitable for residue determination of diazepam in pork.