| 铁矿尾矿放线菌分离株的系统发育分析 |
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| 引用本文： | 吕志堂,刘江,张维维.铁矿尾矿放线菌分离株的系统发育分析[J].安徽农业科学,2010,38(7):3350-3350,3353. |
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| 作者姓名： | 吕志堂 刘江 张维维 |
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| 作者单位： | 河北大学生命科学学院,河北省微生物多样性研究与应用重点实验室,河北保定,071002;河北大学生命科学学院,河北省微生物多样性研究与应用重点实验室,河北保定,071002;河北大学生命科学学院,河北省微生物多样性研究与应用重点实验室,河北保定,071002 |
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| 基金项目： | 国家自然科学基金项目(30800003);;河北大学省基金预研项目(2006Y09) |
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| 摘 要： | 目的]通过确定12株典型分离株的系统发育地位,初步探讨铁矿尾矿中放线菌生物多样性。方法]采用酚-氯仿法提取基因组DNA,PCR扩增16S rDNA并测序后进行系统发育分析。结果]12株铁矿尾矿放线菌均属于链霉菌属,与进化关系最近的已知菌株同源性为98.7%～100%。结论]铁矿尾矿中可培养放线菌类群以链霉菌为主,其中存在大量潜在的新种。
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| 关 键 词： | 铁矿尾矿 放线菌 系统发育分析 链霉菌 |
Phylogenetic Analysis of Actinobacteria Isolates from Iron Mine Tailings |
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| LV Zhi-tang et al.Phylogenetic Analysis of Actinobacteria Isolates from Iron Mine Tailings[J].Journal of Anhui Agricultural Sciences,2010,38(7):3350-3350,3353. |
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| Authors: | LV Zhi-tang |
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| Institution: | College of Life Science;Hebei University;Key Lab of Microbial Diversity Research and Application of Hebei Province;Baoding;Hebei 071002 |
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| Abstract: | Objective] The aim was to discuss the actinobacteria biodiversity of iron-mine tailings by phylogenetic analysis of 12 typical isolates.Method] The genomic DNAs were extracted by phenol-chloroform method;phylogeny analysis was carried out based on 16S rDNA PCR amplification and sequencing.Result] The results showed that all the 12 strains belong to the genus Streptomyces sharing 98.7-99.9% similarities with their nearest known neighbors.Conclusion] Streptomyces is the dominant culturable actinobacteria ... |
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| Keywords: | Iron mine tailings Actinobacteria Phylogenetic analysis Streptomyces |
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