星斑川鲽自交F_1和杂交F_1代免疫相关酶的初步分析

目的]研究星斑川鲽（Platichthys stellatus）自交F1代与星斑川鲽（Platichthys stellatus）♀×圆斑星鲽（Verasper variegates）♂杂交F1代各组织免疫相关酶的活性。方法]比较星斑川鲽F1与杂交F1代肌肉、肝脏、脾脏和肠中3种重要的非特异免疫相关酶：酸性磷酸酶（ACP）、碱性磷酸酶（AKP）和超氧化物歧化酶（SOD）的活性差异。结果]杂交后代肌肉、脾脏、肠中ACP,AKP的活力都高于自交后代,肝脏中ACP,AKP的活性与自交后代无显著差异,SOD活性除肌肉组织中稍增加外,其他组织显著下降。结论]该研究结果为星斑川鲽的杂交选育提供了理论依据。

Analysis on Activity of Immunity-related Enzymes in First Familiar Generation and Self Cross F1 of Platichthys stellatus Pallas

Objective] The aim was to study the activity of immunity-related enzymes in each tissue of self cross F1 of Platichthys stellatus Pallas and first familiar generation of P.stellatus ♀ ×Verasper variegates ♂.Method] The activity difference of three important non-specific immunity-related enzymes: acid phosphatase(ACP),alkaline phosphatase(AKP) and superoxide dismutase(SOD) existed in muscle,liver,spleen and intestine of first familiar generation and self cross F1 of P.stellatus was compared.Result] The activity of ACP and AKP in muscle,liver and intestine of first familiar generation was higher than that of self cross F1,while the activity of ACP and AKP in liver of first familiar generation showed no significant difference with that of self cross F1;the SOD activity decreased significantly in tissues expected for muscle.Conclusion] The result had provided theoretic basis for the breeding of P.stellatus.