女贞不同种质材料基因组 DNA 的提取及 RAPD 引物筛选

目的]提取女贞不同种质材料的基因组DNA，并筛选RAPD引物。方法]采用改良CTAB法提取女贞基因组DNA，并以此种方法提取的女贞基因组DNA为模板，对RAPD引物进行PCR扩增，筛选有效引物。结果]筛选出21个多态性丰富、条带清晰且重复性好的有效引物，经检测所获得的基因组DNA条带清晰，且OD260／DD280在1．8左右。用筛选出的21个有效引物对126份女贞种质材料进行RAPD—PCR扩增，均可获得带型丰富且清晰可辨的DNA指纹图谱。结论]该方法为快速和准确地应用RAPD方法分析女贞种质材料的遗传多样性提供了依据。

Study on the Extraction of Genomic DNA and Screening of RAPD Primer in Different Germplasm Materials of Ligustrum lucidum Ait.

Objective ] To extract genomic DNA in different germplasm materials of Ligustrum lucidum and select RAPD primers. Method ] Genomic DNA was extracted by improved CTAB method, and with genomic DNA as a template, PCR amplification was conducted on RAPD primers. Result ] 21 effective primers with abundant polymorphism, clear bands and good repeatability were obtained. After testing the privet genomic DNA bands were clear, and the OD26o/OD2so was about 1.8. 21 selected effective primers were used in the RAPD-PCR amplification for 126 germplasm materials of L. lucidum. From all of which the abundant and distinct DNA fingerprinting could be obtained. Conclusion] The study will provide a reference for the rapid and accurate utilization RAPD method in analyzing genetic diversity of the germplasm materials of L. lucidum.