由孤雌生殖诱导系诱导的玉米单倍体的鉴定方法 |
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引用本文: | 魏俊杰,陈梅香,刘志增,祝丽英.由孤雌生殖诱导系诱导的玉米单倍体的鉴定方法[J].安徽农业科学,2010,38(18):9429-9430,9469. |
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作者姓名: | 魏俊杰 陈梅香 刘志增 祝丽英 |
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作者单位: | 保定学院,河北保定,071000;河北农业大学农学院,河北保定,071001 |
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基金项目: | 保定市教育科学"十一五"规划课题 |
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摘 要: | 目的]介绍由孤雌生殖诱导系诱导的玉米单倍体的鉴定方法。方法]利用孤雌生殖诱导系诱导的单倍体为材料,根据籽粒Navajo标记和ABP1紫色植株显性双标记系统对诱导产生的单倍体进行鉴定,对根尖细胞染色体数目进行显微观察。结果]籽粒Nav-ajo标记和ABP1紫色植株标记可淘汰大量杂交二倍体,细胞学鉴定可直接验证其他表型鉴别方法的准确性。结论]以上方法对于孤雌生殖单倍体的鉴别是可靠的。这些单倍体鉴别方法各有优缺点,综合利用它们可以提高单倍体鉴定效率。
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关 键 词: | 玉米 孤雌生殖 单倍体 鉴定 |
Identification Method of Maize Monoploid Induced by Parthenogenesis Inducer |
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Institution: | WEI Jun-jie et al (Baoding University,Baoding,Hebei 071000) |
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Abstract: | Objective] The aim was to set up an identification method for maize monoploid induced by parthenogenesis inducer.Method] By using monoploid induced by parthenogenesis inducer as material,the induced monoploid was identified on the basis of grain-Navajo marker and ABP1 purple plant-dominant double markers system and the chromosome number in root-tip cells was observed through microscope.Result] A lot of hybrid diploids could be eliminated by using grain-Navajo marker and ABP1 purple plant-markers.The accuracies of the other phenotype-identification methods could be verificated directly through cytological identification.Conclusion] The identifications by using the above methods on parthenogenesis monoploid were reliable.These monoploid-identification methods had their peculiar advantages and disadvantages and the monoploid-identification efficiency could be enhanced by utilizing them comprehensively. |
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Keywords: | Maize Parthenogenesis Monoploid Identification |
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