哈茨木霉发酵条件的初步研究

目的]了解哈茨木霉30371在自然情况下的培养条件。方法]将哈茨木霉30371的孢子悬液在液体培养基中摇床振荡培养,研究培养温度、pH值、无机离子、各种碳源和氮源等条件对其生长的影响。结果]麦麸培养基中哈茨木霉30371的菌丝干重最高,其次是玉米粉培养基、PDA培养基,豆饼粉培养基最低。哈茨木霉30371在22~34℃下均能生长,22℃为其最适生长温度;在pH值4.0~8.5均能生长,最适pH值是5.5。各因素对哈茨木霉30371生长的影响由大到小依次为:(NH4)2SO4>KH2PO4>MgSO4.7H2O>麦麸、豆饼粉。结论]哈茨木霉最适的发酵条件为:在麦麸培养基中培养,培养温度22℃,自然pH值,2.00%麦麸,1.00%豆饼粉,1.00%(NH4)2SO4,0.25%MgSO4.7H2O,0.50%KH2PO4。

Preliminary Study on Fermentation Conditions of Trichoderma harzianum

Objective] The study was to understand the culture conditions of Trichoderma harzianum 30371 under natural condition.Method] The spore suspension of T.harzianum 30371 was put in liquid medium for shaking culture to study the effects of culture conditions such as the temperature,pH value,inorganic ions,various carbon sources and nitrogen sources etc.on its growth.Result] The mycelial dry weight of T.harzianum 30371 was highest in wheat bran medium,the second was that in maize powder medium and PDA medium,the lowest was that in bean cake powder medium.T.harzianum 30371 could grow under 22-34 ℃,and the optimum growth temperature was 22 ℃.It could grow under pH of 4.0-8.5,and the optimum pH was 5.5.The effects of these conditions on the growth of T.harzianum 30371 from big to small was(NH4)2SO4 dosage>KH2PO4 dosage>MgSO4·7H2O dosage>wheat bran and bean cake powder dosage.Conclusion] The best suitable fermentation conditions of T.harzianum were: culturing in wheat bran medium,culture temperature of 22 ℃,natural pH,2.00% wheat bran,1.00% bean cake powder,1.00%(NH4)2SO4,0.25%MgSO4·7H2O and 0.50% KH2PO4.