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鸡MC4R基因新突变位点的发现
引用本文:陶勇,王金玉,李国辉,胡玉萍.鸡MC4R基因新突变位点的发现[J].安徽农业科学,2008,36(11):4528-4530.
作者姓名:陶勇  王金玉  李国辉  胡玉萍
作者单位:1. 扬州大学动物科学与技术学院,江苏扬州,225009;江苏畜牧兽医职业技术学院,江苏泰州,225300
2. 扬州大学动物科学与技术学院,江苏扬州,225009
基金项目:江苏省高新技术项目(BG2004316);江苏省"青蓝工程"培养基金资助项目.
摘    要:目的]为深入研究鸡MC4R基因和分子育种提供参考。方法]采用饱和酚/氯仿法从鸡血中提取DNA,利用PCR-SSCP和DNA测序的方法,对京海黄鸡MC4R基因的单核苷酸多态性进行分析。结果]对PCR产物进行SSCP分析,2对引物均表现出多态性。引物A得到2种基因型(AA型和AB型)。引物Z也得到2种基因型(CC型和CD型)。将测序结果与发表的鸡MC4R基因序列的比较结果进行比较,发现2个单核苷酸多态位点,其中A引物扩增序列中的多态位点为662 bp处G→C的点突变造成的,而Z引物扩增序列中的多态位点是由于在733~734 bp插入了一个C碱基引起的。结论]该研究中未检测到BB和DD纯合基因型,这可能与在京海黄鸡培育过程中采用分子标记方法进行选择有关。

关 键 词:京海黄鸡  MC4R基因  SNPs
文章编号:0517-6611(2008)11-04528-02
修稿时间:2008年2月13日

Discovery of New Mutation Points in Chicken MC4R Gene
TAO Yong.Discovery of New Mutation Points in Chicken MC4R Gene[J].Journal of Anhui Agricultural Sciences,2008,36(11):4528-4530.
Authors:TAO Yong
Abstract:Objective] The research aimed to provide reference for further study on chicken MC4R gene and its molecular breeding.Method] DNA was extracted from chicken blood by using saturated hydroxybenzene/ chloroform method.And the single nucleotide polymorphism of MC4R gene in Jinghai yellow chicken was analyzed by using the methods of PCR-SSCP and DNA sequencing.Result] The PCR products were analyzed by SSCP and 2 pairs of primers showed the polymorphism.Two kinds of genotypes(AA type and AB type) were obtained from primer A and 2 kinds of genotypes(CC type and CD type) were obtained from primer Z.The comparison results between the sequencing results and the reported MC4R gene sequences showed that 2 single nucleotide polymorphic loci were found.The polymorphic loci in the amplified sequences of primer A was caused by point mutation from G to C at 662 bp and that in the amplified sequences of primer was caused by inserting one C base at 733-734 bp.Conclusion] In this study,pure genotypes BB and DD were not detected,which was probably related with the molecular marker method used in the breeding of Jinghai yellow chicken.
Keywords:Jinghai yellow chicken  MC4R gene  SNPs
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