酶法水解螺旋藻蛋白研究

目的]研究木瓜蛋白酶对螺旋藻蛋白的水解作用。方法]采用正交试验设计分析温度、酶浓度、pH值、水解时间对水解度的影响,利用葡聚糖凝胶柱Sephadex G-25层析测定螺旋藻多肽分子量。结果]木瓜蛋白酶水解螺旋藻蛋白的最适宜工艺参数为:温度55℃,酶与底物比0.4%,时间3 h,pH值6.0。结论]当水解度为82.6%时,2个螺旋藻多肽洗脱峰的分子量分别为2 992和231 Da。

Study on the Enzymatic Hydrolysis of Spirulina Protein

Objective] The aim of the research was to study the hydrolysis of papain on spirulina protein.Method] The effects of temperature,enzyme concentrate,pH value,time on enzymatic hydrolysis were studied by orthogonal design.The molecular weight of the peptide was determind using sephadex G-25.Result] The optimum technological parameters of the hydrolysis of papain on spirulina protein was,temperature 55 ℃,enzyme-substrate ratio 0.4%,time 1 h,pH value 6.0.Conclusion]When the degree of hydrolysis was 82.6%,the molecular weights of two elution peak of peptides were 2 992 and 231 Da.