香菇·灵芝菌丝体多糖的提取及复合抗氧化活性研究

目的]为更好地利用香菇、灵芝菌丝体多糖提供科学依据。方法]采用液体深层发酵获得香菇、灵芝菌丝体,通过正交试验确定香菇、灵芝菌丝体多糖最佳提取条件,并研究2种多糖复合后抗氧化活性。结果]香菇菌丝体胞内多糖的最佳提取条件为料液比1∶10,浸提时间4.0 h,浸提温度90℃,该条件下所提取多糖含量最高为10.3%;灵芝菌丝体胞内多糖的最佳提取条件为料液比1∶20,浸提时间3.5 h,浸提温度90℃,该条件下所提取多糖含量最高为17.2%。香菇菌丝体多糖∶灵芝菌丝体多糖为1∶1的复合多糖对羟自由基的清除效果最好,最高可达62.89%,比单味多糖提高50%以上。结论]香菇、灵芝菌丝体多糖经过合适的配伍可显著提高自由基清除效果。

Study on the Extraction of Lentinus edodes and Ganoderma lucidum Mycelia Polysaccharides and the Compound Anti-oxidative Activity

Objective] The scientific basis of better utilization of Lentinus edodes and Ganoderma lucidum mycelia polysaccharides was provided through the research.Method] The Lentinus edodes and Ganoderma lucidum mycelia was produced by way of the submerged fermentation method.The optimal extraction conditions of polysaccharides were investigated with the orthogonal test and also the anti-oxidative activity of two polysaccharides after their compound was studied.Results] The optimal extraction conditions of intrac...