| 抑制差减杂交法克隆小麦抗病基因相关片段研究 |
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| 引用本文： | 刘志祥,曾超珍,张增艳.抑制差减杂交法克隆小麦抗病基因相关片段研究[J].安徽农业科学,2007,35(26):8128-8129. |
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| 作者姓名： | 刘志祥 曾超珍 张增艳 |
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| 作者单位： | 中南林业科技大学生命科学与技术学院,湖南长沙,410004;中国农业科学院作物科学研究所,北京,100081 |
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| 基金项目： | 中南林业科技大学引进高层次人才科研启动基金（101-0254）. |
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| 摘 要： | 以YW642/中8601的F2代纯合的抗病和感病单株各10株提取DNA构建成近等基因池,以抗病池为试验方,以感病池为驱动方,进行抑制差减杂交(SSH),构建差减文库。结果表明,用差异筛选排除假阳性,得到8个阳性克隆;Southern杂交表明,克隆TSH-1具有抗病池特异性,且为寡拷贝或单拷贝序列;经测序,TSH-1长388bp,提交GenBank进行Blast比对,没有找到与之同源的序列。
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| 关 键 词： | 抑制差减杂交 小麦 抗病基因 |
| 文章编号： | 0517-6611（2007）26-08128-02 |
| 修稿时间： | 2007-04-26 |
Cloning of Fragments Related to Disease Resistance Genes in Wheat by Suppression Subtractive Hybridization |
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| LIU Zhi-xiang et al.Cloning of Fragments Related to Disease Resistance Genes in Wheat by Suppression Subtractive Hybridization[J].Journal of Anhui Agricultural Sciences,2007,35(26):8128-8129. |
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| Authors: | LIU Zhi-xiang |
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| Institution: | College of Life Science;Central South University of Forestry and Technology;Changsha;Hunan 410004 |
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| Abstract: | Near isogenic pools was constructed by mixing 10 resistant individual plant of F2 generation of YW642/Zhong 8601 as resistant pool and 10 susceptible individual plant as susceptible pool. Suppression subtractive hybridization (SSH) was carried out by using resistant pool as tester and susceptible pool as driver and SSH DNA library was constructed. The forward and reverse SSH products were used as probe to screen the library to remove the false positive clones. 8 positive clones were screened out and one clone TSH-1 was proved to be special to resistant pool by southern hybridization. The sequence of the clone was queried to GenBank but no similar sequence was found. |
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| Keywords: | Suppression subtractive hybridization Wheat Disease resistance gene |
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