利用多重荧光PCR分析秦川牛遗传多样性

目的]分析秦川牛的遗传多样性,加强对该品种资源的保护。方法]利用15个微卫星座位,运用多重荧光PCR技术对59头秦川牛个体的基因组DNA进行扩增,通过等位基因频率、多态信息含量、基因杂合度和有效等位基因数分析秦川牛品种的遗传多样性。结果]结果表明:在15个座位的等位基因数为5～18个,平均等位基因数为10.3个;各座位上的杂合度都较高,平均杂合度为0.7959;平均有效等位基因数为5.3066;15个座位中多态信息含量为0.6441～0.8649,均为高度多态。结论]通过荧光标记可用于牛品种遗传多样性的分析,并可为进一步QTL定位和标记辅助选择研究提供参考。

Analysis of Genetic Diversity of Qinchuan Cattle by Multiplex Fluorescent PCR

Objective The aim was to study the genetic diversity of Qinchuan cattle in order to strengthen its protection.Method The genetic diversity of 15 microsatellite loci was analyzed using multiplex fluorescent PCR in 59 Qinchuan cattle.And allele frequency,polymorphism information content(PIC),heterozygosity(H) and number of effective alleles(Ne) were calculated.Result The results showed that the allele numbers of the overall 15 microsatellite loci of Qinchuan cattle were from 5 to 18 with average allele of 10.3.The heterozygosity of all microsatellite loci were from 0.698 6 to 0.876 3 and the mean heterozygosity were 0.795 9.The number of effective alleles was from 3.317 8 to 8.084 5,with average effective allele of 5.306 6.The PIC of 15 microsatellite loci were highly polymorphic from 0.644 1 to 0.864 9.Conclusion Microsatellite markers were effective markers for analysis of genetic diversity and phylogenesis relationship among cattle breeds.The results were reference to study QTL and marker assistant selection.