广东省狂犬病病毒分离株N基因的序列分析 |
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引用本文: | 陈晶,袁洁,柯昌文,谷文喜,黄元,徐敏,陈少勤,张健騑,宣华,向华.广东省狂犬病病毒分离株N基因的序列分析[J].安徽农业科学,2010,38(34):19539-19540. |
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作者姓名: | 陈晶 袁洁 柯昌文 谷文喜 黄元 徐敏 陈少勤 张健騑 宣华 向华 |
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作者单位: | [1]广东省农业科学院兽医研究所,广东广州510640 [2]广东省疾病预防控制中心,广东广州510300 [3]中山大学公共卫生学院,广东广州510080 |
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基金项目: | 动物狂犬病监测、防制技术研究与示范项目,公共实验室开放基金项目 |
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摘 要: | 目的]确定广东地区狂犬病毒流行毒株,更好地防控狂犬病。方法]对2009年从广东省各地采集的犬脑和对应唾液腺进行直接免疫荧光和巢式RT-PCR检测,并通过颅内接种乳鼠进一步鉴定毒株。PCR扩增分离株的N基因,测定其核苷酸序列,并将其与广西地区的流行毒株和疫苗株进行了比对。结果]分离到2株狂犬病毒毒株,其N基因序列与广西分离到的病毒具有极高的相似性(97.9%~99.4%),与狂犬病毒疫苗株的相似性为87.7%~88.1%。结论]外表健康犬只可能携带狂犬病毒,且狂犬病毒的流行存在明显的地域差异。
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关 键 词: | 狂犬病毒 N基因 序列分析 |
Sequence Analyses on N Gene of Rabies Viruses Isolated from Guangdong Province |
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Institution: | CHEN Jing et al(Veterinary Medicine Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510640) |
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Abstract: | Objective] To find the dominant rabies virus strains in Guangdong Province and thus to better prevent and control rabies.Method] Canine brains and salivary glands collected in several regions of Guangdong Province were detected by direct immunofluorescence assay and nested RT-PCR.The isolates were further identified by intracranial inoculation in suckling mice.The N gene was amplified by RT-PCR and the sequences were aligned with those of prevalent strains in Guangxi region and vaccine strains.Result] Two strains were isolated from Guangdong Province.Their N gene sequences had high similarity of 97.9%-99.4% to those of Guangxi strains and also had similarity of 87.7%-88.1% to those of vaccine strains of rabies virus.Conclusion] Apparently healthy dogs may carry rabies virus,and the prevalence of rabies virus shows obvious regional differences. |
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Keywords: | Rabies virus N gene Sequence analyses |
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