| 亚硝化作用菌种的分离筛选及条件选择 |
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| 引用本文： | 廖雪义,马光庭,蓝荣,李献.亚硝化作用菌种的分离筛选及条件选择[J].安徽农业科学,2007,35(5):1259-1261. |
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| 作者姓名： | 廖雪义 马光庭 蓝荣 李献 |
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| 作者单位： | 1. 襄樊学院化学与生物科学系,湖北襄樊,441053;2. 广西大学农学院,广西南宁,530005;3. 广东省中山市杨氏南北鲜果有限公司,广东中山,528451 |
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| 摘 要： | 通过富集培养、硅胶平板分离法和亚硝化作用试验,筛选出1株亚硝化速率较高的菌株(编号为N4,下同).经初步鉴定其为亚硝化单胞菌(Nitrosospira sp.),并对它作了亚硝化作用条件试验.试验结果表明:其最佳的亚硝化作用条件为温度30 ℃、pH值7.5～8.0,初始氨氮浓度100～150 mg/L,通气量为摇床转速110 r/min,碱度为NaHCO3浓度1 700 mg/L.在此条件下,接种浓度为15%,培养24 h,氨氮去除率为99.47%,亚硝酸盐氮积累量可达到116.65 mg/L.
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| 关 键 词： | 亚硝化作用 亚硝化细菌 菌种分离筛选 条件选择 |
| 文章编号： | 0517-6611（2007）05-01259-03 |
| 修稿时间： | 2006-11-14 |
Study on Isolation Screening and Condition Selection of Nitrobacteria |
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| LIAO Xue-yi et al.Study on Isolation Screening and Condition Selection of Nitrobacteria[J].Journal of Anhui Agricultural Sciences,2007,35(5):1259-1261. |
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| Authors: | LIAO Xue-yi |
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| Institution: | Department of Chemistry and Biology, Xiangfan University, Xiangfan, Hubei 441053 |
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| Abstract: | A nitrobacteria of high nitrosification rate was isolated in this paper.After preliminary examination,it was concluded that the bacteria was Nitrosospira sp.Results showed that the optimal nitrosification condition was 25~30 ℃,pH 7.5～8.0,110 r/min,1 700 mg/L NaHCO3 alkalinity,150 mg/L NH4+-N.Under the above condition and 15 % inoculation rate,after 24 h,the removal efficiency of NH4+-N reached 99.47 %,and accumulative volume of NO2--N was 116.65 mg/L. |
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| Keywords: | Nitrosification Nitrite bacteria Bacteria screening Conditional selection |
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