| 草本刺嫩芽根多糖脱蛋白方法研究 |
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| 引用本文： | 李峰,刘延吉,宗绪岩,于瑞洪,祝寰宇.草本刺嫩芽根多糖脱蛋白方法研究[J].安徽农业科学,2006,34(1):9-10. |
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| 作者姓名： | 李峰 刘延吉 宗绪岩 于瑞洪 祝寰宇 |
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| 作者单位： | 沈阳农业大学生物技术与科学学院,辽宁,沈阳,100116;沈阳农业大学生物技术与科学学院,辽宁,沈阳,100116;沈阳农业大学生物技术与科学学院,辽宁,沈阳,100116;沈阳农业大学生物技术与科学学院,辽宁,沈阳,100116;沈阳农业大学生物技术与科学学院,辽宁,沈阳,100116 |
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| 摘 要： | 采用正交试验探讨三氯乙酸S、evag、蛋白质等电点等方法去除草本刺嫩芽多糖中的蛋白质杂质。试验结果表明:草本刺嫩芽多糖脱蛋白的最佳条件为三氯乙酸浓度为9%,pH值为8,Sevag法次数为3次,其中1 g中含蛋白质为0.173%,最终多糖得率为12.85%。
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| 关 键 词： | 草本刺嫩芽 多糖 脱蛋白 |
| 文章编号： | 0517-6611（2006）01-0009-02 |
| 收稿时间： | 2005-08-29 |
| 修稿时间： | 2005-08-29 |
Study on Elimination Protein from the Roofs of Aralia continentalis Kitag Polysaccharide |
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| LI Feng et al.Study on Elimination Protein from the Roofs of Aralia continentalis Kitag Polysaccharide[J].Journal of Anhui Agricultural Sciences,2006,34(1):9-10. |
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| Authors: | LI Feng |
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| Institution: | Biotechnology and Scienee College,Shenyang Agricultural University,Shenyang,Liaoning 110161 |
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| Abstract: | It is an important step in the distillation of polysaccharide to eliminate protein that can directly influence to the analysis of polysaccharide.To remove impure protein in polysacchari-de liquid in Aralia continentalis Kitag with the different methods of TCA,Sevag,Isoelectric point of protein and the methods were optimized by orthogonal experiment.The results were as follows: the TCA concentration 9%,pH 8 and Sevag times 3 are optimum condition,among them containing protein as 1.734?mg/g and polysaccharide as 12.85%. |
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| Keywords: | Aralia continentalis Kitag Polysaccharide Elimination protein |
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