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PCR法检测水中金黄色葡萄球菌肠毒素A基因
引用本文:叶云,钟英英.PCR法检测水中金黄色葡萄球菌肠毒素A基因[J].安徽农业科学,2008,36(26).
作者姓名:叶云  钟英英
作者单位:广西工学院生物与化学工程系,广两柳州,545006
摘    要:目的]建立一种快速准确检测水中金黄色葡萄球菌肠毒素A的方法。方法]将金黄色葡萄球菌模拟污染的水增菌后提取菌体DNA,以沙门氏菌、大肠杆菌DNA和空白作对照,用PCR法扩增金黄色葡萄球菌肠毒素A基因。结果]金黄色葡萄球菌DNA的检测结果呈阳性,检测底限达100 cfu/L,而大肠杆菌和沙门氏菌及空白对照均未出现特异性片段,整个检测过程只需要24 h。结论]试验建立的PCR方法可检测水及类似食品中的金黄色葡萄球菌SEA基因,并具有特异性强、灵敏度高、速度快和易操作的特点。

关 键 词:金黄色葡萄球菌  肠毒素A基因  PCR技术  水样

Study on the Detection of Staphylococcus aureus Enterotoxin A Gene in Water by PCR
YE Yun et al.Study on the Detection of Staphylococcus aureus Enterotoxin A Gene in Water by PCR[J].Journal of Anhui Agricultural Sciences,2008,36(26).
Authors:YE Yun
Abstract:Objective] The aim was to establish a rapid specific assay for the detection of Staphylococcus aureu enterotoxin A in drinking water.Method] A polymerase chain reaction(PCR)assay targeting gene encoding Staphylococcus aureus enterotoxin A was developed for detecting Staphylococcus aureus in pure DNA and artificially contaminated water.Salmonella,E.coli DNA and blank were also amplified under the same condition.Staphylococcus aureus enterotoxin A was amplified by PCR.Result] The detection result of Staphylococcus aureus DNA was positive,with detection limit being 100 cfu/L;the while PCR products was not found in Salmonella,E.coli and blank.The total assay time including enrichment was approximately 24 h.Conclusion] Results suggested that the PCR could be used to rapidly detect Staphylococcus aureus in water and possibly other types of food products,and the PCR assay also showed high specificity,sensitivity and easy operation.
Keywords:Staphylococcus aureus  Enterotoxin A gene  PCR technology  Water sample
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