低温胁迫对葡萄幼苗相关生理指标的影响

目的]探究低温胁迫对葡萄幼苗相关生理指标的影响。方法]选用早黑宝葡萄试管幼苗为材料,当长至5~6片真叶时挑选长势相同的葡萄幼苗进行常温25(CK)1、5、5℃3种不同温度处理,于处理后0、24、、68、d进行各项生理指标测定,之后恢复常温处理,即恢复2 d(R2)4、d(R4),测定相关生理指标,研究不同程度低温处理对葡萄试管幼苗形态指标和生理指标(叶绿素含量、可溶性糖含量S、OD活性、POD活性、MDA活性及质膜相对透性(PMP))的影响。结果]葡萄试管幼苗经低温胁迫后,叶绿素含量下降,可溶性糖含量升高;SODP、OD活性先升后降;低温造成葡萄试管苗的膜脂过氧化,MDA含量升高、PMP增大。结论]15℃低温处理对葡萄试管幼苗的伤害是可逆的,而5℃处理造成了不可逆伤害。

Effects of Low Temperature Stress on the Related Physiological Indexes in Grape Seedlings

Objective] The research aimed to explore the effects of low temperature stress on the related physiological indexes in grape seedlings.Method] Using the seedlings of Zaoheibao grape in vitro as materials,the grape seedlings with 5～6 euphylla and the same growth vigor were treated with 3 kinds of temperature including normal temperature(25 ℃ as CK),15 ℃and 5 ℃. All the physiological indexes were determined on 0 th,2 nd,4 th,6 th,8 th after the treatments.And then the treatment of normal temperature was resumed for 2 d(R2) and 4 d(R4) to determine the related physiological indexes.The effects of different degree of low temperature treatments on the morphological indexes and the physiological indexes(chlorophyll content,the content of soluble sugar,SOD activity,POD activity、MDA activity and relative permeability of plasma membrane(PMP)) of grape seedlings in vitro were studied.Result] After low temperature stress,chlorophyll content of grape seedlings in vitro was decreased and the content of soluble sugar was increased,and the activities of SOD and POD were first increased and then decreased.Low temperature caused the peroxidation of membrane lipid in grape seedlings in vitro,the increase of MDA content and the enlargement of PMP.Conclusion] The injury of low temperature treatment at 15 ℃ to grape seedlings in vitro was reversible and the treatment at 5 ℃ caused the irreversible injury.