魔芋葡甘聚糖的磷酸酯化改性研究

目的]研发新型的生物可降解凝胶。方法]以魔芋葡甘聚糖为主要原料、三聚磷酸钠为交联剂,合成了生物可降解的磷酸酯化魔芋葡甘聚糖水凝胶,并探讨了该水凝胶的溶胀动力学以及不同反应条件对凝胶平衡溶胀比的影响,并通过体外降解试验分析了该水凝胶的生物可降解性。结果]该水凝胶在溶胀初期溶胀比迅速增加,随着溶胀时间的延长,溶胀比增长逐渐变慢,8 h后达到溶胀平衡。该水凝胶的平衡溶胀比随魔芋葡甘聚糖用量的增加而逐渐升高,随着交联剂三聚磷酸钠用量的增加而逐渐降低。该水凝胶可被含有β-葡聚糖苷酶的纤维素酶降解,而不能被不含β-葡聚糖苷酶的胰酶等降解,保持了魔芋葡甘聚糖所具有的生物可降解性。结论]磷酸酯化魔芋葡甘聚糖水凝胶可作为用于结肠定位释药的生物可降解水凝胶。

Research on the Modification of Konjak Glucomannan with Phosphate Esterification

The aim of the research was to develop new type of biodegradable gel.With konjak glucomannan as main materials and sodium tripolyphosphate as crosslinking agent,the biodegradable konjak glucomannan hydrogel with phosphate esterification was synthesized.And the swelling dynamics of this hydrogel and the effects of different reaction conditions on the equilibrium swelling ratio of the hydrogel were discussed.The biodegradability of the hydrogel was analyzed by in vitro degradation test.The swelling ratio of the hydrogel in the early stage was increased rapidly.And the increasing of swelling ratio was gradually slowed with the prolonging of the swelling time and it reached swelling equilibrium after 8 h.The equilibrium swelling ratio of the hydrogel was gradually increased with the increasing of konjak glucomannan dosage and was gradually decreased with the increasing of the dosage of crosslinking agent sodium tripolyphosphate.The hydrogel could be degraded by cellulose that contained β-glucanase.But it couldn't be degraded by pancreatic enzyme that didn't contain β-glucanase and it maintained the biodegradability of konjak glucomannan.Konjak glucomannan can be used as biodegradable hydrogel for colon site-specific drug delivery.