转双价基因(Bt+CpTI)抗虫棉对棉蚜羧酸酯酶和乙酰胆碱酯酶比活力的影响

目的]研究了转双价基因棉SGK321对棉蚜Aphis gossypii Glover羧酸酯酶和乙酰胆碱酯酶比活力的影响,为转基因棉的生物安全性评价提供理论依据。方法]在SGK321及其亲本棉石远321上长期饲养棉蚜,研究2种棉花品种对棉蚜短期（第1、2、3代）和长期（第41、42、43代）的影响。结果]在SGK321上取食1代的棉蚜种群羧酸酯酶比活力显著高于在亲本棉上取食1、2、3代的棉蚜种群,转双价基因棉SGK321上饲养1、2、3代的棉蚜乙酰胆碱酯酶比活力均显著高于在石远321上饲养相同代数的棉蚜。但长期饲养的2种棉蚜之间羧酸酯酶比活力、乙酰胆碱酯酶比活力无显著差异。结论]长期取食转双价基因棉SGK321的棉蚜通过棉蚜解毒酶的调节作用对SGK321产生了适应性。

Effect of Transgenic Bt plus CpTI Cotton on Carboxylesterase and Acetylcholinesterase of Cotton Aphid Aphis gossypii

Objective] The research aimed to assess the effect of transgenic Bt plus CpTI cotton variety SGK321 on carboxylesterase and acetylcholinesterase of cotton aphid Aphis gossypii and give theoretical basis for studying the biosafety of transgenic cotton.Method] Cotton aphids were fed with both SGK321 and Shiyuan321(normal parental varieties) for over 40 generations.Enzyme activities were compared between cotton aphids fed on SGK321 for 1,2,3,41,42 and 43 generations with those on Shiyuan321.Result] The carboxylesterase activities of cotton aphids fed on SGK321 for 1 generation was significantly higher than those fed on Shiyuan321.Acetylcholinesterase activities of cotton aphids fed on SGK321 for 1,2 and 3 generations were significantly higher than those fed on Shiyuan321 in the same generation.But there was no significant difference of enzyme activities among cotton aphids fed on SGK321 for long run,and those on parental cotton.Conclusion] The cotton aphid that fed on transgenic Bt plus CpTI cotton SGK321 for the long run have applicability about SGK321 through the accommodation of Detoxifying Enzyme.