Al^3＋对蚯蚓和家兔乙酰胆碱酯酶的毒性研究

目的]探讨Al3＋对蚯蚓和家兔乙酰胆碱酯酶的毒性。方法]通过体外试验,分别研究了Al3＋对蚯蚓乙酰胆碱酯酶（AChE）和家兔脑部AChE的毒力（IC50）和双分子速率Ki的影响。结果]Al3＋对蚯蚓AChE和家兔脑部AChE的IC50分别为5.4和4.2 mmol/L;4.2mmol/LAl3＋对家兔大脑AChE的双分子速率Ki值为5.4mmol/LAl3＋对蚯蚓AChE双分子速率Ki值的1.02倍,表明家兔脑部AChE对A l3＋的敏感性较蚯蚓AChE对Al3＋的敏感性强。结论]为从整个动物界层面阐明铝对AChE的毒性性质以及利用AChE快速检测铝的污染提供了理论依据。

Toxicity of Aluminum on Acetylcholinesterase(AChE) of Earthworm and Habit

Objective] The aim was to study the toxicity of aluminum on acetylcholinesterase(AChE) of earthworm and habit.Method] The toxicity of aluminum on AChE of habit brain and earthworm were evaluated in vitro.Result] AChE activities were depressed significantly by concentration ranges of Al3+.The IC50 values of Al3+ on AChE activity of earthworm and habit brain were 5.4 and 4.2 mmol/L,respectively.The bimolecular rate constant(Ki) of 4.2 mmol/L Al3+ on AChE of habit brain was 1.02 times more than that of 5.4 mmol/L Al3+ on AChE of earthworm,which suggested that AChE from habit brain has higher sensitivity to Al3+ than that of earthworm.Conclusion] The research provides theoretical basis for clarifying the toxicity of aluminum on AchE from the whole of animal life layer and fastly determining the pollution of aluminum by AchE.