| 紫色链霉菌产褐藻胶裂解酶培养条件的初步研究 |
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| 引用本文： | 刘航,曹海龙,李曙光,杜昱光.紫色链霉菌产褐藻胶裂解酶培养条件的初步研究[J].安徽农业科学,2011,39(22):13282-13284. |
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| 作者姓名： | 刘航 曹海龙 李曙光 杜昱光 |
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| 作者单位： | 中国科学院大连化学物理研究所生物技术部,辽宁大连116023;中国科学院研究生院,北京100049;中国科学院研究生院,北京,100049 |
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| 基金项目： | 国家高技术研究发展计划项目(2011AA090704); 中国科学院知识创新工程重要方向项目(KSCX2-YW-G-041); 公益性(农业)科研专项(200903052); 重大新药创制(2009-ZX09501-011) |
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| 摘 要： | 目的]初步研究紫色链霉菌产褐藻胶裂解酶的最佳培养条件。方法]以一株具有较高褐藻胶裂解酶活力的紫色链霉菌为出发菌株,考察了底物浓度、氮源、培养温度、培养时间、装液量、KH2PO4浓度、MgSO4.7H2O浓度对产酶的影响。结果]最佳产酶条件：以蛋白胨和酵母提取物为氮源1,.4%褐藻胶0,.4%KH2PO40,.06%MgSO4.7H2O,培养温度30℃,培养时间6 d。结论]该研究为紫色链霉菌的发酵产酶及褐藻胶裂解酶的制备奠定了基础。
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| 关 键 词： | 紫色链霉菌 褐藻胶裂解酶 培养条件 |
Initial Research on the Culture Condition of Alginate Lyase Production by Streptomyces violaceoruber |
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| LIU Hang et al.Initial Research on the Culture Condition of Alginate Lyase Production by Streptomyces violaceoruber[J].Journal of Anhui Agricultural Sciences,2011,39(22):13282-13284. |
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| Authors: | LIU Hang |
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| Institution: | LIU Hang et al(Biotechnology Department,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian,Liaoning 116023) |
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| Abstract: | Objective] The research aimed to study initially the best culture condition of alginate lyase production by Streptomyces violaceoruber.Method] Streptomyces violaceoruber whose alginate lyase activity was high was as the initial strain.The influences of substrate concentration,nitrogen source,culture temperature,culture time,liquid volume,KH2PO4 concentration,MgSO4·7H2O concentration on the enzyme production were inspected.Result] The best enzyme production condition: peptone and yeast extract were as the nitrogen source,1.4% alginate,0.4% KH2PO4,0.06% MgSO4·7H2O,the culture temperature was 30 ℃,and the culture time was 6 d.Conclusion] The research laid the foundation for the fermentation,enzyme production of Streptomyces violaceoruber and the preparation of alginate lyase. |
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| Keywords: | Streptomyces violaceoruber Alginate lyase Culture condition |
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