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NaCl胁迫对甘薯试管苗生长及离子含量影响
引用本文:高叶,赵术珍,陈敏,宋晓征,王宝山.NaCl胁迫对甘薯试管苗生长及离子含量影响[J].安徽农业科学,2008,36(35).
作者姓名:高叶  赵术珍  陈敏  宋晓征  王宝山
作者单位:山东师范大学生命科学学院逆境植物重点实验室,山东济南,250014
基金项目:国家自然科学基金,山东省优秀中青年科学家科研奖励基金
摘    要:目的]探讨甘薯的耐盐机理。方法]用浓度分别为0、100 mmol/LNaCl处理耐盐性不同的2个甘薯品种徐25-2(耐盐品种)和胜利百号(盐敏感品种),培养20 d后以火焰光度计测定根、茎、叶中的Na+含量、Na+/K+比值,并测不同品种的根、茎、叶的干重、鲜重。结果]盐胁迫下,2个甘薯品种的生长均受抑制,导致植株矮小、叶片变少、根系稀少、根叶干物质减少,但是徐25-2幼苗受抑制程度较轻;此外,2个甘薯品种不同器官(根、茎、叶)的Na+含量及Na+/K+比值都增加,耐盐性强的徐25-2的Na+含量在根、茎和叶片中较低,而耐盐性较弱的胜利百号幼苗茎、叶Na+含量较高,但徐25-2的变化幅度均小于胜利百号。结论]盐胁迫下叶片较低的Na+含量和Na+/K+比值是甘薯品种耐盐性的重要特征。

关 键 词:盐胁迫  甘薯  试管苗  生长  离子含量  耐盐性

Effect of Sodium Chloride Stress on Growth of Sweet Potato Plantlets in vitro and Ion Content
Abstract:Objective] The aim of this study was to investigate the salt-tolerance mechanism of sweet potato.Method] Two sweet potato varieties of Xu 25-2(salt-tolerant cultivar) and Triumph 100(salt-sensitive cultivar) were treated by sodium chloride with the concentration of 0 and 100 mmol/L.After 20 days,Na+ content and Na+/K+ ratio in the roots,shoot and leave were determined by the flame photometer,while dry weight and fresh weight of roots,shoot and leave in different varieties were also studied.Result] The growth of two sweet potato varieties was inhibited under salt stress,so the plant became shorter,leaf and root became fewer,dry weight of roots and leave decreased,but seedlings of Xu 25-2 were inhibited slightly.Furthermore,Na+ content and Na+/K+ ratio in roots,shoot and leaves of two sweet potato varieties increased.Na+ content of salt-tolerant Xu 25-2 was low in roots,shoot and leaves,while Na+ content of salt-sensitive Triumph 100 was high in shoot and leave of seedlings,but the change range of Xu 25-2 was less than that of Triumph 100.Conclusion] The lower Na+ content and Na+/K+ ratio in leaves under salt stress is the most important characteristics for salt-tolerance of sweet potato varieties.
Keywords:Salt stress  Sweet potato  Plantlets in vitro  Growth  Ion content  Salt tolerance
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