| 植物乳杆菌IMAU10116产生抗娄地青霉活性物质培养基的优化 |
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| 引用本文： | 戚薇,石景,尹娅凤,王海宽.植物乳杆菌IMAU10116产生抗娄地青霉活性物质培养基的优化[J].安徽农业科学,2011(19). |
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| 作者姓名： | 戚薇 石景 尹娅凤 王海宽 |
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| 作者单位： | 工业发酵微生物教育部重点实验室天津科技大学生物工程学院; |
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| 基金项目： | 国家自然科学基金项目(20876116、30900961); 乳品生物技术与工程教育部重点实验室开放课题基金项目 |
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| 摘 要： | 目的]筛选对娄地青霉有较强抑菌活性的植物乳杆菌并提高其抗真菌活性。方法]将活化后的植物乳杆菌IMAU10116接种到MRS液体培养基中培养,采用双层平板点接法评价其抗娄地青霉菌的活性。结果]单因素试验表明,以葡萄糖为碳源时,植物乳杆菌IMAU10116对娄地青霉的抑制效果最好;植物乳杆菌以麦芽浸粉为氮源时,抑菌效果最好;抗真菌乳酸菌产生抗菌物质的最佳生长因子为玉米浆粉。植物乳杆菌IMAU10116最佳培养基组成为:葡萄糖20g/L+麦芽浸粉20g/L+玉米浆粉7g/L+适量无机盐。用优化培养基培养的植物乳杆菌IMAU10116抑制娄地青霉菌产生的抑菌圈为18.6mm,用MRS培养基所产生的抑菌圈为15.0mm。结论]植物乳杆菌IMAU10116对娄地青霉有很好的抑制作用,有望在食品及饲料添加剂领域获得较好的应用。
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| 关 键 词： | 植物乳杆菌 培养基优化 抗真菌 娄地青霉 |
Optimization of Medium for Lactobacillus plantarum IMAU10116 Producing Active Compound against Penicillium roqueforti |
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| QI Wei et al.Optimization of Medium for Lactobacillus plantarum IMAU10116 Producing Active Compound against Penicillium roqueforti[J].Journal of Anhui Agricultural Sciences,2011(19). |
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| Authors: | QI Wei |
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| Institution: | QI Wei et al(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Bioengineering,Tianjin University of Science and Technology,Tianjin 300457) |
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| Abstract: | Objective]The study aimed to screen out Lactobacillus plantarum possessing higher inhibitory activity against Penicillium roqueforti and enhance its antifungal activity.Method]The activated L.plantarum IMAU10116 was innoculated MRS liquid medium for culturing and double-layer plate point inoculation was used to evaluate its activity against P.roqueforti.Result]The single-factor experiments showed that when glucose was used as carbon source,the inhibitory effect of L.plantarum IMAU10116 against P.roquefor... |
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| Keywords: | Lactobacillus plantarum Medium optimization Antifungal Penicillium roqueforti |
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