菊花多糖提取工艺研究

目的]确定菊花多糖的提取、醇沉以及纯化的最佳条件。方法]以多糖得率为指标,采用正交试验对菊花多糖的提取、醇沉及纯化工艺进行优选。结果]菊花多糖水提工艺因素影响顺序：提取次数〉固液比〉提取温度〉提取时间;提取工艺为：固液比20∶1（V∶M,g/ml）,提取时间4h,提取温度95℃,提取3次。醇沉工艺因素影响顺序：醇沉时间〉乙醇体积分数〉药液浓缩程度;醇沉工艺为：乙醇体积分数80%,药液的浓缩程度1∶3,醇沉时间9h。纯化工艺因素影响顺序：氯仿∶正丁醇〉纯化时间〉样品∶氯仿-正丁醇;纯化工艺为：氯仿与正丁醇的配比5∶1,样品和氯仿-正丁醇的体积比2∶1,纯化时间10min。结论]该工艺适合菊花多糖的提取。

Study on the Technique of Extracting Polysaccharide from Chrysanthemum morifolium Ramat

Objective]The research aimed to confirm the best extraction,alcohol precipitation and purification conditions of polysaccharide in Chrysanthemum morifolium Ramat.Method]With the yield of polysaccharides as an index,the extraction,alcohol precipitation and purification processes of polysaccharide in C.morifolium were optimized.Result]The influence sequence of the factors of water extraction of poly-saccharide from C.morifolium was as follows:extraction times>solid-liquid ratio>extraction temperature>extraction time.The best exraction process was as follows:solid-liquid ratio of 20∶1(V∶M,g/ml) ,extraction time of 4 h,extracting at 95 ℃ for 3 times.The influencing sequence of alcohol precipitation process was alcohol precipitation time>ethanol volume fraction>concentrated degree of medicine liquid.The alcohol precipitation process was as follows:ethanol volume fraction of 80 % ,concentrated degree of medicine liquid of 1∶ 3,alcohol precipitation time of 9 h.The influencing factors sequence of purification technology was as follows:chloroform/n-butanol > purification time>samples/chloroform+n-butanol.The purification process was as follows:chloroform/n-butanol of 5∶1,the volume ratio of samples to chloroform and n-butanol of 2∶1,purification time of 10 min.Conclusion]The process was suitable for the extraction of polysaccharide from C.morifolium.