化学诱变枯草芽孢杆菌对大白菜根肿病的防治效果

目的]研究化学诱变枯草芽孢杆菌对大白菜根肿病的防治效果.方法]采用3株来自枯草芽孢杆菌XF-1的化学诱变菌株XF-1A、XF-1C和XF-1E进行大白菜根肿病的盆栽防病试验,并以MALDI-TOF-MS分析了3种抗生素的相对含量.结果]菌株XF-1A和XF-1C防效较好,分别为68.80％和65.06％,XF-1E防效较差为42.21％.3菌株均能分泌表面活性素、伊枯草菌素和丰源素,但表面活性素和伊枯草菌素相对含量为XF-1A＞ XF-1C＞ XF-1E,XF-1A的表面活性素和伊枯草菌素分别达29.26％和35.31％,XF-1E的丰源素含量最高,达41.09％.结论]表面活性素和伊枯草菌素含量与对根肿病防治效果存在一定的关联性

Control Effect of Chemically Induced Bacillus subtilis on Chinese Cabbage Clubroot Disease

Objective] The aim was to study control effect of chemically induced Bacillus subtilis on Chinese cabbage clubroot disease. Method] Three mutants, XF-1 A, XF-1C and XF-1E chemically induced from a wild strain, Bacillus subtilis XF-1, were used to control clubroot disease of Chinese cabbage in pot experiment and to analyze their antibiotics by MALDI-TOF-MS technique. Result ] The results showed that XF-1A and XF-1C had 68.80% and 65.06% control effect, respectively, and XF-1E had 42.20% control effect. These strains could produce surfactin, iturin and fengycin. But they produced the relative amount of iturin and surfactin in order of XF-1A 〉 XF-1C 〉 XF- 1E. XF-1A contained 29.26% and 35.31% of surfactin and iturin, XF-1E had highest amount of 41.09% fengycin. Conclusion] Surfactin and iturin may be associated with the disease control effect.