不同基原金线莲植物的化学成分比较研究

目的]比较不同基原金线莲植物的化学成分.方法]应用LC-MS联用技术对福建金线莲(Anoectochilus roxburghii)、台湾金线莲(A.formosanus)组培植株和福建金线莲林下种植植株进行比较分析,同时应用硫酸-蒽酮法和分光光度法对金线莲主要活性成分(金线莲多糖、总黄酮)的含量进行比较分析研究.结果]福建金线莲(A.roxburghii)、台湾金线莲(4.formosanus)组培植株和福建金线莲林下种植植株3个金线莲样品(HNJ201001、HNJ201002和HNJ201003)中金线莲多糖的含量分别为16.175％、19.636％和10.939％；3个金线莲样品中总黄酮的含量分别为0.722％、0.895％和0.984％.结论]3个样品的化学成分组成及含量非常相似,其中总黄酮和游离氨基酸的含量均与文献报道的野生金线莲相当或略高,而组培金线莲样品中的多糖则远高于文献报道的野生金线莲样品.

Comparative Study of the Chemical Constituents of Anoectochilus spp. from different origins

Objective] To compare the chemical constituents of Anoectochilus spp.(Jin-Xian-Lian) from different origins.Method] The chemical constituents of three Jin-Xian-Lian samples were analyzed and compared by means of LC-MS techniques,which were the tissue cultured A.roxburghii and A.formosanus,and A.roxburghii planted under forest.At the same time,the contents of the main active components in Jin-Xian-Lian were compared and analyzed by anthrone-sulfuric acid method and ultraviolet spectrophotometry,including polysaccharides and total flavonoids.Result] Polysaccharides contents in three Jin-Xian-Lian samples(HNJ201001,HNJ201002 and HNJ201003) were 16.175%,19.636% and 10.939%;while the total flavonoids contents were 0.722%,0.895% and 0.984%,respectively.Conclusion] The results showed that the chemical composition and their content of these three samples were quite similar.Of which,the contents of total flavonoids and free amino acids of the three Jin-Xian-Lian samples were similar to or even higher than those of the wild species.As for the tissue cultured Jin-Xian-Lian,polysaccharides content was far higher than the wild species.