荧光茧色判性家蚕黄酮合酶Ⅰ(BmFNS Ⅰ)基因在家蚕组织内的表达差异 |
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引用本文: | 许乃霞,张雨青,沈卫德.荧光茧色判性家蚕黄酮合酶Ⅰ(BmFNS Ⅰ)基因在家蚕组织内的表达差异[J].安徽农业科学,2011(1):41-43. |
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作者姓名: | 许乃霞 张雨青 沈卫德 |
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作者单位: | 苏州大学基础医学与生物科学学院;苏州大学蚕桑研究所 |
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基金项目: | 国家重点基础研究发展计划“863”项目(2006AA10A118);江苏省高校自然科学基金项目(07KJB230102) |
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摘 要: | 采用荧光定量PCR分别检测不同品种荧光判性家蚕雌、雄家蚕的中肠、丝腺和脂肪体中的黄酮合酶I(BmFNS I)基因的表达。结果表明,家蚕BmFNS I基因在各品种荧光判性家蚕中肠中的相对表达量最高,其次是脂肪体,在丝腺中的表达量较低,说明BmFNSI基因在对家蚕食物桑叶内的黄酮类化合物的合成代谢中起重要作用;但是在各品种荧光判性家蚕的雌、雄中肠中的表达并无明显差异,说明荧光判性家蚕雌、雄蚕在对桑叶内的黄酮类化合物的合成代谢途径上不存在差异,至少在FNS I基因所调节的黄酮类化合物的合成代谢上不存在差异。
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关 键 词: | 荧光茧色判性 家蚕 黄酮合酶Ⅰ 组织 |
Expression Differences of Flavonoid Synthase Ⅰ(BmFNS Ⅰ) in the Tissues of Bombyx Mori with Sex-identified Fluorescent Cocoon Color |
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Institution: | XU Nai-xia et al(School of Basic Medicine and Biological Sciences,Soochow University,Suzhou,Jiangsu 215123) |
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Abstract: | Expression differences of Flavonoid synthase Ⅰ(BmFNS Ⅰ) gene in the midgut,silk gland and fat body of the male and female Bombyx mori with sex-identified fluorescent cocoon color was detected by fluorescence quantitative PCR. And the result showed that the relative expression level BmFNS Ⅰ gene in the midgut of the silkwormn was the highest,followed by that in fat body,and the expression level in silk gland was the lowest,indicating that BmFNS Ⅰ gene played an important role in flavonoids anabolism in leaves of mulberry,which was the food of silkworm. However,there was no significant difference in the BmFNS Ⅰ gene expression level in the midgut between male and female Bombyx mori varieties with sex-identified fluorescent cocoon color,suggesting that there was no difference in anabolism of flavonoids in leaves between the male and female Bombyx mori with sex-identified fluorescent cocoon color,at least no difference in anabolism of flavonoids regulated by flavonoid synthase Ⅰ gene. |
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Keywords: | Sex-identified fluorescent cocoon color Bombyx mori Flavonoid synthase Ⅰ Tissues |
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