罹病光肩星天牛幼虫分离菌株的16S rDNA系统发育分析

目的]研究从罹病光肩星天牛Anoplophora glabripennis(Motsch.)]幼虫刻槽中分离的菌株BH-1的16S rDNA系统发育及该菌株杀虫特性。方法]常规细菌鉴定,将BH-1接种健康天牛后观察杀虫效果并通过设计特异引物扩增其16S rDNA序列,进行测序及同源性分析。结果]用1010cfu/ml BH-1菌液接种2龄天牛幼虫8 d后致死率达72.7%。BH-1与GenBank收录的Serratia marcescens的16S rDNA序列的同源性达99.5%,同时结合细菌的部分常规鉴定结果,鉴定出BH-1菌株为粘质沙雷氏菌(S.marcescens)。结论]BH-1的发现为天牛的生物防治提供了参考。

Phylogenetic Analysis of the 16S rDNA of a Strain Isolated from Diseased Larva of Anoplophora glabripennis (Motsch.)

Objective] Study on the phylogenetic analysis of the 16S rDNA and insecticidal characteristics of strain BH-1 isolated from diseased larva of Anoplophora glabripennis(Motsch.) Method] The strain was identified by routine method and inoculated onto healthy Anoplophora glabripennis(Motsch.) for observing insecticidal effect,further 16S rDNA was amplified by the specific primers for sequencing and homology analysis.Result] The mortality of second instar of Anoplophora glabripennis(Motsch.) reached 72.7% 8 d after 1010 cfu/ml BH-1 was inoculated.The homology of 16S rDNA sequences between BH-1 and Serratia marcescens accessed in GenBank reached 99.5%.Combined with the results of routine identification,BH-1 was identified as S.marcescens.Conclusion] BH-1 could be used for biological control of Anoplophora glabripennis(Motsch.).