高效液相色谱法测定卷烟主流烟气中甘露糖和葡萄糖含量

目的]建立高效液相色谱测定卷烟主流烟气中甘露糖和葡萄糖含量的方法。方法]采用超声萃取吸附卷烟烟气总粒相物的剑桥滤片,经固相萃取柱纯化,1-苯基-3-甲基-5-吡唑啉酮衍生,高效液相色谱-紫外检测。采用KH2PO4-NaOH(0.05 mol/L,pH=6.8)/乙腈=85/15等梯度洗脱,在HP Hypersil ODS(125×4 mmi.d.,5μm)柱上检测了两种单糖成分。结果]甘露糖在0.5~8.0μg/ml、葡萄糖在5~50μg/m l范围内线性关系良好(R2>0.999),检测限均为0.14μg/m l(S/N=3),RSD均小于3%,回收率95.8%~99.8%。结论]该方法能准确地同时检测卷烟烟气中甘露糖和葡萄糖的含量。

Determination of Mannose and Glucose in Mainstream Cigarette Smoke by High Performance Liquid Chromatography

Objective] A chromatographic method was developed to determine mannose and glucose in mainstream cigarette smoke using high-performance liquid chromatography.Method] Mainstream smoke particulate was collected on Cambridge filter pad.The particulate was then extracted in a ultrasonic bath and purified by solid-phase extraction cartridge.The extract was derivatized with 1-phenyl-3-methyl-5-pyrazolone(PMP) and detected with ultraviolet detector.Two monosaccharide derivatives were successfully detected on HP Hypersil ODS(125×4 mm i.d.,5 μm) column using equivalent elution with the mobile phase of 0.05 mol/L phosphate buffer(pH 6.8)-acetonitrile(85∶ 15).Result] There was well linear relationship of two monosaccharides in the range of 0.5-8.0 μg/ml for mannose and 5-50 μg/ml for glucose respectively(R2>0.999).The detection limits were both 0.14 μg/ml(S/N=3).The RSD(n=5) of both monosaccharides were below 3%.In addition,the spiked recoveries were between 95.8%-99.8%.Conclusion] The results indicated that this method was reliable for the determination of mannose and glucose in mainstream cigarette smoke.